RGD Reference Report - Efficient and rapid generation of large genomic variants in rats and mice using CRISMERE. - Rat Genome Database

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Efficient and rapid generation of large genomic variants in rats and mice using CRISMERE.

Authors: Birling, Marie-Christine  Schaeffer, Laurence  André, Philippe  Lindner, Loic  Maréchal, Damien  Ayadi, Abdel  Sorg, Tania  Pavlovic, Guillaume  Hérault, Yann 
Citation: Birling MC, etal., Sci Rep. 2017 Mar 7;7:43331. doi: 10.1038/srep43331.
RGD ID: 14929209
Pubmed: PMID:28266534   (View Abstract at PubMed)
PMCID: PMC5339700   (View Article at PubMed Central)
DOI: DOI:10.1038/srep43331   (Journal Full-text)

Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of genomic regions has been labour intensive and time consuming. We developed the CRISpr MEdiated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate most of the desired rearrangements from a single experiment at much lower expenses and in less than 9 months. Deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse founders were observed and germ line transmission was confirmed for fragment as large as 3.6 Mb. Interestingly we have been able to recover duplicated regions from founders in which we only detected deletions. CRISMERE is even more powerful than anticipated it allows the scientific community to manipulate the rodent and probably other genomes in a fast and efficient manner which was not possible before.

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
decreased body weight  IMP 14929209 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Dyrk1a  (dual specificity tyrosine phosphorylation regulated kinase 1A)


Additional Information