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Noninvasive imaging of in vivo MuRF1 expression during muscle atrophy.

Authors: Li, Wei  Claypool, Mark D  Friera, Annabelle M  McLaughlin, John  Baltgalvis, Kristen A  Smith, Ira J  Kinoshita, Taisei  White, Kathy  Lang, Wayne  Godinez, Guillermo  Payan, Donald G  Kinsella, Todd M 
Citation: Li W, etal., PLoS One. 2014 Apr 7;9(4):e94032. doi: 10.1371/journal.pone.0094032. eCollection 2014.
Pubmed: (View Article at PubMed) PMID:24710205
DOI: Full-text: DOI:10.1371/journal.pone.0094032

Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic research in this effort is important for advancing this goal. In this report, we describe the development of a bioluminescent gene reporter rat, based on the zinc finger nuclease-targeted insertion of a bicistronic luciferase reporter into the 3' untranslated region of a muscle specific E3 ubiquitin ligase gene, MuRF1 (Trim63). In longitudinal studies, we noninvasively assess atrophy-related expression of this reporter in three distinct models of muscle loss (sciatic denervation, hindlimb unloading and dexamethasone-treatment) and show that these animals are capable of generating refined detail on in vivo MuRF1 expression with high temporal and anatomical resolution.

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RGD Object Information
RGD ID: 14695084
Created: 2019-07-03
Species: All species
Last Modified: 2019-07-03
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.