RGD Reference Report - Stereochemistry of dehydrogenation catalyzed by Acyl-CoA oxidase. - Rat Genome Database

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Stereochemistry of dehydrogenation catalyzed by Acyl-CoA oxidase.

Authors: Kawaguchi, A  Tsubotani, S  Seyama, Y  Yamakawa, T  Osumi, T  Hashimoto, T  Kikuchi, T  Ando, M  Okuda, S 
Citation: Kawaguchi A, etal., J Biochem. 1980 Nov;88(5):1481-6. doi: 10.1093/oxfordjournals.jbchem.a133118.
RGD ID: 14402446
Pubmed: (View Article at PubMed) PMID:7462191
DOI: Full-text: DOI:10.1093/oxfordjournals.jbchem.a133118

The stereochemical course of dehydrogenation catalyzed by acyl-CoA oxidase was investigated using the enzymes from rat liver peroxisomes and Candida lipolytica. Stearoyl-CoA and nonanoyl-CoA, stereospecifically labeled with deuterium at either C-2 or C-3, were incubated with the enzyme, the products were converted to methyl esters and their deuterium contents were measured by gas chromatography-mass spectrometry. The results suggested that acyl-CoA oxidase-catalyzed dehydrogenation occurred by anti-elimination of the pro 2R and pro-3R hydrogens of acyl-CoA.



Gene Ontology Annotations    

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Acox1  (acyl-CoA oxidase 1)


Additional Information