RGD Reference Report - Efficient liver repopulation of transplanted hepatocyte prevents cirrhosis in a rat model of hereditary tyrosinemia type I. - Rat Genome Database

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Efficient liver repopulation of transplanted hepatocyte prevents cirrhosis in a rat model of hereditary tyrosinemia type I.

Authors: Zhang, Ludi  Shao, Yanjiao  Li, Lu  Tian, Feng  Cen, Jin  Chen, Xiaotao  Hu, Dan  Zhou, Yan  Xie, Weifen  Zheng, Yunwen  Ji, Yuan  Liu, Mingyao  Li, Dali  Hui, Lijian 
Citation: Zhang L, etal., Sci Rep. 2016 Aug 11;6:31460. doi: 10.1038/srep31460.
RGD ID: 14398823
Pubmed: (View Article at PubMed) PMID:27510266
DOI: Full-text: DOI:10.1038/srep31460

Hereditary tyrosinemia type I (HT1) is caused by a deficiency in the enzyme fumarylacetoacetate hydrolase (Fah). Fah-deficient mice and pigs are phenotypically analogous to human HT1, but do not recapitulate all the chronic features of the human disorder, especially liver fibrosis and cirrhosis. Rats as an important model organism for biomedical research have many advantages over other animal models. Genome engineering in rats is limited till the availability of new gene editing technologies. Using the recently developed CRISPR/Cas9 technique, we generated Fah(-/-) rats. The Fah(-/-) rats faithfully represented major phenotypic and biochemical manifestations of human HT1, including hypertyrosinemia, liver failure, and renal tubular damage. More importantly, the Fah(-/-) rats developed remarkable liver fibrosis and cirrhosis, which have not been observed in Fah mutant mice or pigs. Transplantation of wild-type hepatocytes rescued the Fah(-/-) rats from impending death. Moreover, the highly efficient repopulation of hepatocytes in Fah(-/-) livers prevented the progression of liver fibrosis to cirrhosis and in turn restored liver architecture. These results indicate that Fah(-/-) rats may be used as an animal model of HT1 with liver cirrhosis. Furthermore, Fah(-/-) rats may be used as a tool in studying hepatocyte transplantation and a bioreactor for the expansion of hepatocytes.

Disease Annotations    
liver cirrhosis  (IMP,ISO)
Liver Failure  (IMP,ISO)
tyrosinemia type I  (IMP,ISO)

Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

Genes (Rattus norvegicus)
Fah  (fumarylacetoacetate hydrolase)
Fahem15Dlli-/-  (fumarylacetoacetate hydrolase; CRISPR/Cas9 induced mutant 15, Dlli)

Genes (Mus musculus)
Fah  (fumarylacetoacetate hydrolase)

Genes (Homo sapiens)
FAH  (fumarylacetoacetate hydrolase)

SD-Fahem15Dlli-/-  (NA)

Additional Information