RGD Reference Report - Neuroglycan C is a novel midkine receptor involved in process elongation of oligodendroglial precursor-like cells. - Rat Genome Database

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Neuroglycan C is a novel midkine receptor involved in process elongation of oligodendroglial precursor-like cells.

Authors: Ichihara-Tanaka, Keiko  Oohira, Atsuhiko  Rumsby, Martin  Muramatsu, Takashi 
Citation: Ichihara-Tanaka K, etal., J Biol Chem. 2006 Oct 13;281(41):30857-64. doi: 10.1074/jbc.M602228200. Epub 2006 Aug 10.
RGD ID: 14397555
Pubmed: PMID:16901907   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M602228200   (Journal Full-text)

Midkine is a heparin-binding growth factor that promotes cell attachment and process extension in undifferentiated bipolar CG-4 cells, an oligodendroglial precursor cell line. We found that CG-4 cells expressed a non-proteoglycan form of neuroglycan C, known as a part-time transmembrane proteoglycan. We demonstrated that neuroglycan C before or after chondroitinase ABC treatment bound to a midkine affinity column. Neuroglycan C lacking chondroitin sulfate chains was eluted with 0.5 m NaCl as a major fraction from the column. We confirmed that CG-4 cells expressed two isoforms of neuroglycan C, I, and III, by isolating cDNA. Among three functional domains of the extracellular part of neuroglycan C, the chondroitin sulfate attachment domain and acidic amino acid cluster box domain showed affinity for midkine, but the epidermal growth factor domain did not. Furthermore, cell surface neuroglycan C could be cross-linked with soluble midkine. Process extension on midkine-coated dishes was inhibited by either a monoclonal anti-neuroglycan C antibody C1 or a glutathione S-transferase-neuroglycan C fusion protein. Finally, stable transfectants of B104 neuroblastoma cells overexpressing neuroglycan C-I or neuroglycan C-III attached to the midkine substrate, spread well, and gave rise to cytoskeletal changes. Based on these results, we conclude that neuroglycan C is a novel component of midkine receptors involved in process elongation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoskeleton organization involved_inIMP 14397555PMID:16901907UniProt 
glial cell projection elongation involved_inIDA 14397555PMID:16901907UniProt 
positive regulation of substrate adhesion-dependent cell spreading involved_inIMP 14397555PMID:16901907UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P1202514397555PMID:16901907UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Cspg5  (chondroitin sulfate proteoglycan 5)


Additional Information