RGD Reference Report - Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras. - Rat Genome Database

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Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras.

Authors: Stahl, B  Chou, J H  Li, C  Südhof, T C  Jahn, R 
Citation: Stahl B, etal., EMBO J. 1996 Apr 15;15(8):1799-809.
RGD ID: 14397552
Pubmed: PMID:8617225   (View Abstract at PubMed)
PMCID: PMC450096   (View Article at PubMed Central)

GTP activates the interaction between the synaptic vesicle proteins rabphilin and rab3. This raises the question of whether rabphilin is a resident vesicle protein that recruits rab3 in a stage-dependent fashion, or if it is instead an effector protein recruited by rab3. We now show that rabphilin, like rab3, dissociates from synaptic vesicles after exocytosis in a manner requiring both Ca2+ and membrane fusion. Rabphilin interacts with GTP-rab3 via a N-terminal domain comprising a novel Zn2+(-)finger motif, and this interaction is essential for rabphilin binding to synaptic vesicles. Thus, in the same way that ras recruits raf to the plasma membrane, rab3 reversibly recruits rabphilin to synaptic vesicles in a stage-dependent manner. These results reveal an unexpected similarity between the molecular mechanisms by which small G protein function in recruiting effector proteins to membranes during membrane traffic and signal transduction.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
synaptic vesicle located_inIDA 14397552; 14397552PMID:8617225UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P4770914397552PMID:8617225UniProt 
protein binding enablesIPIUniProtKB:P6301214397552PMID:8617225UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Rab3a  (RAB3A, member RAS oncogene family)
Rph3a  (rabphilin 3A)


Additional Information