RGD Reference Report - Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack. - Rat Genome Database

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Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack.

Authors: Volchuk, Allen  Ravazzola, Mariella  Perrelet, Alain  Eng, William S  Di Liberto, Maurizio  Varlamov, Oleg  Fukasawa, Masayoshi  Engel, Thomas  Söllner, Thomas H  Rothman, James E  Orci, Lelio 
Citation: Volchuk A, etal., Mol Biol Cell. 2004 Apr;15(4):1506-18. doi: 10.1091/mbc.e03-08-0625. Epub 2004 Jan 23.
RGD ID: 14394613
Pubmed: (View Article at PubMed) PMID:14742712
DOI: Full-text: DOI:10.1091/mbc.e03-08-0625

Genetic and biochemical evidence has established that a SNARE complex consisting of syntaxin 5 (Sed5)-mYkt6 (Ykt6)-GOS28 (Gos1)-GS15 (Sft1) is required for transport of proteins across the Golgi stack in animals (yeast). We have utilized quantitative immunogold labeling to establish the cis-trans distribution of the v-SNARE GS15 and the t-SNARE subunits GOS28 and syntaxin 5. Whereas the distribution of the t-SNARE is nearly even across the Golgi stack from the cis to the trans side, the v-SNARE GS15 is present in a gradient of increasing concentration toward the trans face of the stack. This contrasts with a second distinct SNARE complex, also required for intra-Golgi transport, consisting of syntaxin 5 (Sed5)-membrin (Bos1)-ERS24 (Sec22)-rBet1 (Bet1), whose v-(rBet1) and t-SNARE subunits (membrin and ERS24), progressively decrease in concentration toward the trans face. Transport within the stack therefore appears to utilize countercurrent gradients of two Golgi SNAREpins and may involve a mechanism akin to homotypic fusion.



Gene Ontology Annotations    

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Bet1  (Bet1 golgi vesicular membrane trafficking protein)
Bet1l  (Bet1 golgi vesicular membrane trafficking protein-like)


Additional Information