RGD Reference Report - Formation of Golgi-derived active zone precursor vesicles. - Rat Genome Database
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Formation of Golgi-derived active zone precursor vesicles.

Authors: Maas, Christoph  Torres, Viviana I  Altrock, Wilko D  Leal-Ortiz, Sergio  Wagh, Dhananjay  Terry-Lorenzo, Ryan T  Fejtova, Anna  Gundelfinger, Eckart D  Ziv, Noam E  Garner, Craig C 
Citation: Maas C, etal., J Neurosci. 2012 Aug 8;32(32):11095-108. doi: 10.1523/JNEUROSCI.0195-12.2012.
RGD ID: 14390063
Pubmed: (View Article at PubMed) PMID:22875941
DOI: Full-text: DOI:10.1523/JNEUROSCI.0195-12.2012

Vesicular trafficking of presynaptic and postsynaptic components is emerging as a general cellular mechanism for the delivery of scaffold proteins, ion channels, and receptors to nascent and mature synapses. However, the molecular mechanisms leading to the selection of cargos and their differential transport to subneuronal compartments are not well understood, in part because of the mixing of cargos at the plasma membrane and/or within endosomal compartments. In the present study, we have explored the cellular mechanisms of active zone precursor vesicle assembly at the Golgi in dissociated hippocampal neurons of Rattus norvegicus. Our studies show that Piccolo, Bassoon, and ELKS2/CAST exit the trans-Golgi network on a common vesicle that requires Piccolo and Bassoon for its proper assembly. In contrast, Munc13 and synaptic vesicle proteins use distinct sets of Golgi-derived transport vesicles, while RIM1α associates with vesicular membranes in a post-Golgi compartment. Furthermore, Piccolo and Bassoon are necessary for ELKS2/CAST to leave the Golgi in association with vesicles, and a core domain of Bassoon is sufficient to facilitate formation of these vesicles. While these findings support emerging principles regarding active zone differentiation, the cellular and molecular analyses reported here also indicate that the Piccolo-Bassoon transport vesicles leaving the Golgi may undergo further changes in protein composition before arriving at synaptic sites.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Bsn  (bassoon (presynaptic cytomatrix protein))
Erc2  (ELKS/RAB6-interacting/CAST family member 2)
Pclo  (piccolo (presynaptic cytomatrix protein))
Sv2a  (synaptic vesicle glycoprotein 2a)
Unc13a  (unc-13 homolog A)


Additional Information