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Disruption of the murine intestinal alkaline phosphatase gene Akp3 impairs lipid transcytosis and induces visceral fat accumulation and hepatic steatosis.

Authors: Nakano, Takanari  Inoue, Ikuo  Koyama, Iwao  Kanazawa, Kenta  Nakamura, Koh-Ichi  Narisawa, Sonoko  Tanaka, Kayoko  Akita, Masumi  Masuyama, Taku  Seo, Makoto  Hokari, Shigeru  Katayama, Shigehiro  Alpers, David H  Millán, José Luis  Komoda, Tsugikazu 
Citation: Nakano T, etal., Am J Physiol Gastrointest Liver Physiol. 2007 May;292(5):G1439-49. doi: 10.1152/ajpgi.00331.2006. Epub 2007 Mar 1.
Pubmed: (View Article at PubMed) PMID:17332477
DOI: Full-text: DOI:10.1152/ajpgi.00331.2006

Intestinal alkaline phosphatase (IAP) is involved in the process of fat absorption, a conclusion confirmed by an altered lipid transport and a faster body weight gain from 10 to 30 wk in both male and female mice with a homozygous null mutation of the IAP coding gene (Akp3(-/-) mice). This study was aimed to delineate morphologically and quantitatively the accelerated lipid absorption in male Akp3(-/-) mice. Feeding a corn oil bolus produced an earlier peak of triacylglycerol in serum (2 vs. 4 h for Akp3(-/-) and wild-type mice, respectively) and an approximately twofold increase in serum triacylglycerol concentration in Akp3(-/-) mice injected with a lipolysis inhibitor, Triton WR-1339. A corn oil load induced the threefold enlargement of the Golgi vacuoles in male wild-type mice but not in Akp3(-/-) mice, indicating that absorbed lipids rarely reached the Golgi complex and that the transcytosis of lipid droplets does not follow the normal pathway in male Akp3(-/-) mice. Force feeding an exaggerated fat intake by a 30% fat chow for 10 wk induced obesity in both male Akp3(-/-) and wild-type mice, and therefore no phenotypic difference was observed between the two. On the other hand, the forced high-fat chow induced an 18% greater body weight gain, hepatic steatosis, and visceral fat accumulation in female Akp3(-/-) mice but not in female wild-type controls. These results provide further evidence that IAP is involved in the regulation of the lipid absorption process and that its absence leads to progressive metabolic abnormalities in certain fat-forced conditions.


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RGD Object Information
RGD ID: 14349048
Created: 2019-02-11
Species: All species
Last Modified: 2019-02-11
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.