RGD Reference Report - Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-alpha biosynthesis in rat astrocytes. - Rat Genome Database

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Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-alpha biosynthesis in rat astrocytes.

Authors: Sun, Lin-lin  Cheng, Chun  Liu, Hai-ou  Shen, Cong-cong  Xiao, Feng  Qin, Jing  Yang, Jun-ling  Shen, Ai-guo 
Citation: Sun LL, etal., J Mol Neurosci. 2007;32(1):16-24.
RGD ID: 14348969
Pubmed: PMID:17873284   (View Abstract at PubMed)

The protein kinase C (PKC) is known to be a critical component in the signaling cascades that lead to astrocyte-activation. To further understand the mechanism of PKC signaling in astrocyte-activation, we investigated the effect of SSeCKS, a PKC substrate, on LPS-induced cytokine expression in astrocytes by RT-PCR and enzyme-linked immunosorbent assay. Exposure of the cells to LPS induced rapid translocation of SSeCKS to the perinuclear sides, ERK activation and pronounced TNF-alpha production, which can be inhibited by the PKC inhibitor Gö6983. By using siRNA knockdown of SSeCKS expression, LPS-induced signaling events were partly inhibited, including ERK activation, inducible TNF-alpha biosynthesis and secretion. These results suggest that SSeCKS is involved in the LPS-induced TNF-alpha expression in astrocytes mediated by PKC.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akap12Ratpositive regulation of tumor necrosis factor production  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akap12  (A-kinase anchoring protein 12)


Additional Information