RGD Reference Report - Re-expression of AKAP12 inhibits progression and metastasis potential of colorectal carcinoma in vivo and in vitro. - Rat Genome Database

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Re-expression of AKAP12 inhibits progression and metastasis potential of colorectal carcinoma in vivo and in vitro.

Authors: Liu, Weiwei  Guan, Ming  Hu, Tingting  Gu, Xiaoye  Lu, Yuan 
Citation: Liu W, etal., PLoS One. 2011;6(8):e24015. doi: 10.1371/journal.pone.0024015. Epub 2011 Aug 30.
RGD ID: 14348959
Pubmed: PMID:21918680   (View Abstract at PubMed)
PMCID: PMC3168868   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0024015   (Journal Full-text)


BACKGROUND: AKAP12/Gravin (A kinase anchor protein 12) is one of the A-kinase scaffold proteins and a potential tumor suppressor gene in human primary cancers. Our recent study demonstrated the highly recurrent loss of AKAP12 in colorectal cancer and AKAP12 reexpression inhibited proliferation and anchorage-independent growth in colorectal cancer cells, implicating AKAP12 in colorectal cancer pathogenesis.
METHODS: To evaluate the effect of this gene on the progression and metastasis of colorectal cancer, we examined the impact of overexpressing AKAP12 in the AKAP12-negative human colorectal cancer cell line LoVo, the single clone (LoVo-AKAP12) compared to mock-transfected cells (LoVo-CON).
RESULTS: pCMV6-AKAP12-mediated AKAP12 re-expression induced apoptosis (3% to 12.7%, p<0.01), migration (89.6±7.5 cells to 31.0±4.1 cells, p<0.01) and invasion (82.7±5.2 cells to 24.7±3.3 cells, p<0.01) of LoVo cells in vitro compared to control cells. Nude mice injected with LoVo-AKAP12 cells had both significantly reduced tumor volume (p<0.01) and increased apoptosis compared to mice given AKAP12-CON. The quantitative human-specific Alu PCR analysis showed overexpression of AKAP12 suppressed the number of intravasated cells in vivo (p<0.01).
CONCLUSION: These results demonstrate that AKAP12 may play an important role in tumor growth suppression and the survival of human colorectal cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKAP12Humancolorectal carcinoma treatmentIDA  RGD 
Akap12Ratcolorectal carcinoma treatmentISOAKAP12 (Homo sapiens) RGD 
Akap12Mousecolorectal carcinoma treatmentISOAKAP12 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akap12  (A-kinase anchoring protein 12)

Genes (Mus musculus)
Akap12  (A kinase anchor protein 12)

Genes (Homo sapiens)
AKAP12  (A-kinase anchoring protein 12)


Additional Information