RGD Reference Report - 3,5-diiodo-L-thyronine upregulates rat-liver mitochondrial F(o)F(1)-ATP synthase by GA-binding protein/nuclear respiratory factor-2. - Rat Genome Database

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3,5-diiodo-L-thyronine upregulates rat-liver mitochondrial F(o)F(1)-ATP synthase by GA-binding protein/nuclear respiratory factor-2.

Authors: Mangiullo, Roberto  Gnoni, Antonio  Damiano, Fabrizio  Siculella, Luisa  Zanotti, Franco  Papa, Sergio  Gnoni, Gabriele V 
Citation: Mangiullo R, etal., Biochim Biophys Acta. 2010 Feb;1797(2):233-40. doi: 10.1016/j.bbabio.2009.10.009. Epub 2009 Oct 28.
RGD ID: 13830874
Pubmed: PMID:19878644   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbabio.2009.10.009   (Journal Full-text)

Besides triiodothyronine (T3), 3,5-diiodo-L-thyronine (T2) has been reported to affect mitochondrial bioenergetic parameters. T2 effects have been considered as independent of protein synthesis. Here, we investigated the effect of in vivo chronic T2 administration to hypothyroid rats on liver mitochondrial F(o)F(1)-ATP synthase activity and expression. T2 increased state 4 and state 3 oxygen consumption and raised ATP synthesis and hydrolysis, which were reduced in hypothyroid rats. Immunoblotting analysis showed that T2 up-regulated the expression of several subunits (alpha, beta, F(o)I-PVP and OSCP) of the ATP synthase. The observed increase of beta-subunit mRNA accumulation suggested a T2-mediated nuclear effect. Then, the molecular basis underlying T2 effects was investigated. Our results support the notion that the beta-subunit of ATP synthase is indirectly regulated by T2 through, at least in part, the activation of the transcription factor GA-binding protein/nuclear respiratory factor-2. These findings provide new insights into the T2 role on bioenergetic mechanisms.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypothyroidism treatmentISOAtp5po (Rattus norvegicus)13830874; 13830874 RGD 
hypothyroidism treatmentIEP 13830874 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Atp5po  (ATP synthase peripheral stalk subunit OSCP)

Genes (Mus musculus)
Atp5po  (ATP synthase peripheral stalk subunit OSCP)

Genes (Homo sapiens)
ATP5PO  (ATP synthase peripheral stalk subunit OSCP)


Additional Information