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Peripheral gene expression profile of mechanical hyperalgesia induced by repeated cold stress in SHRSP5/Dmcr rats.

Authors: Kozaki, Yasuko  Umetsu, Rena  Mizukami, Yukako  Yamamura, Aya  Kitamori, Kazuya  Tsuchikura, Satoru  Ikeda, Katsumi  Yamori, Yukio 
Citation: Kozaki Y, etal., J Physiol Sci. 2015 Sep;65(5):417-25. doi: 10.1007/s12576-015-0380-9. Epub 2015 May 14.
Pubmed: (View Article at PubMed) PMID:25972297
DOI: Full-text: DOI:10.1007/s12576-015-0380-9

Repeated cold stress (RCS) is known to transiently induce functional disorders associated with hypotension and hyperalgesia. In this study, we investigated the effects of RCS (24 and 4 °C alternately at 30-min intervals during the day and 4 °C at night for 2 days, followed by 4 °C on the next 2 consecutive nights) on the thresholds for cutaneous mechanical pain responses and on peripheral expression of "pain-related genes" in SHRSP5/Dmcr rats, which are derived from stroke-prone spontaneously hypertensive rats. To define genes peripherally regulated by RCS, we detected changes in the expression of pain-related genes in dorsal root ganglion cells by PCR-based cDNA subtraction analysis or DNA microarray analysis, and confirmed the changes by RT-PCR. We found significantly changed expression in eight pain-related genes (upregulated: Fyn, St8sia1, and Tac 1; downregulated: Ctsb, Fstl1, Itpr1, Npy, S100a10). At least some of these genes may play key roles in hyperalgesia induced by RCS.

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RGD ID: 13793383
Created: 2018-10-03
Species: All species
Last Modified: 2018-10-03
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.