RGD Reference Report - Differential expression of the LAMB3 and LAMC2 genes between small cell and non-small cell lung carcinomas. - Rat Genome Database

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Differential expression of the LAMB3 and LAMC2 genes between small cell and non-small cell lung carcinomas.

Authors: Manda, R  Kohno, T  Niki, T  Yamada, T  Takenoshita, S  Kuwano, H  Yokota, J 
Citation: Manda R, etal., Biochem Biophys Res Commun. 2000 Aug 28;275(2):440-5. doi: 10.1006/bbrc.2000.3331.
RGD ID: 13793368
Pubmed: PMID:10964684   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2000.3331   (Journal Full-text)

To identify genes differentially expressed between small cell lung carcinoma (SCLC) cells and non-SCLC cells, mRNA differential display was applied to 3 SCLC cell lines and 6 non-SCLC cell lines. The LAMB3 gene was identified as being expressed only in non-SCLC cells and not in SCLC cells. The LAMB3 gene encodes the laminin beta3 chain, which is a unique component of laminin-5. Laminin-5 is a heterotrimer protein consisting of the alpha3, beta3, and gamma2 chains, and another unique component of laminin-5 is the gamma2 chain encoded by the LAMC2 gene. RT-PCR analysis of the LAMB3 and LAMC2 genes in 45 lung cancer cell lines revealed that both the LAMB3 and LAMC2 genes were co-expressed in 21 of 32 non-SCLC cell lines (66%) but only in one of 13 SCLC cell lines (8%). Coexpression of the LAMB3 and LAMC2 genes was also observed in all 4 cases of primary non-SCLC cells examined but not in the corresponding non-cancerous lung cells. Since alpha6beta4 integrin, the specific laminin-5 binding receptor, is known to be expressed only in non-SCLC cells and not in SCLC cells, it was indicated that laminin-5 is a critical microenvironmental factor for the growth of non-SCLC cells but not of SCLC cells. The differences in the expression of integrins and laminins would be critical factors to distinguish SCLC and non-SCLC cells, and such differences might be associated with the unique biological properties of SCLC cells, including metastatic potential and drug sensitivity.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lung non-small cell carcinoma  IEP 13793368; 13793368mRNA:increased expression:lung:RGD 
lung non-small cell carcinoma  ISOLAMB3 (Homo sapiens)13793368; 13793368mRNA:increased expression:lung:RGD 
lung non-small cell carcinoma  ISOLAMC2 (Homo sapiens)13793368; 13793368mRNA:increased expression:lung:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lamb3  (laminin subunit beta 3)
Lamc2  (laminin subunit gamma 2)

Genes (Mus musculus)
Lamb3  (laminin, beta 3)
Lamc2  (laminin, gamma 2)

Genes (Homo sapiens)
LAMB3  (laminin subunit beta 3)
LAMC2  (laminin subunit gamma 2)


Additional Information