RGD Reference Report - Renoprotective effects of berberine and its possible molecular mechanisms in combination of high-fat diet and low-dose streptozotocin-induced diabetic rats. - Rat Genome Database

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Renoprotective effects of berberine and its possible molecular mechanisms in combination of high-fat diet and low-dose streptozotocin-induced diabetic rats.

Authors: Wang, Feng Ling  Tang, Li Qin  Yang, Feng  Zhu, Ling Na  Cai, Ming  Wei, Wei 
Citation: Wang FL, etal., Mol Biol Rep. 2013 Mar;40(3):2405-18. doi: 10.1007/s11033-012-2321-5. Epub 2012 Nov 30.
RGD ID: 13792785
Pubmed: PMID:23196710   (View Abstract at PubMed)
DOI: DOI:10.1007/s11033-012-2321-5   (Journal Full-text)

Berberine (BBR), an effective compound of Chinese traditional herbal medicine, has preventive effects on diabetes and its complications. In this study, we investigated the therapeutic effects and underlying molecular mechanisms of BBR in rats with high-fat diet and streptozotocin (STZ)-induced diabetic nephropathy model. BBR (50, 100, 200 mg/kg/d) were orally administered to male Sprague-Dawley rats after STZ injection and conducted for 8 weeks. Renal damage was evaluated by kidney weight to body weight ratio (KW/BW), urine microalbumin (UMAlb), urine protein for 24 h (UP24 h), urine creatinine (UCr), and histological examination. Type IV collagen and transforming growth factor-beta1 (TGF-ß1) were detected by immunohistochemistry and ultrastructure of glomeruli was observed. Fasting blood glucose (FBG),serum creatinine (SCr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-c), low-density lipoprotein-cholesterol (LDL-c) in serum and G protein-coupled receptor kinases (GRKs), cAMP in kidney were measured. Remarkable renal damage, hyperglycemia and hyperlipidemia were observed in DN rats. BBR could restore renal functional parameters, suppress alterations in histological and ultrastructural changes in the kidney tissues, improve glucose and lipid metabolism disorders, and increase cAMP levels compared with those of DN model group. Furthermore, BBR down-regulated total protein expression of GRK2, GRK3 and up-regulated expression of GRK6 of renal cortex in DN rats, but had a slight effects on GRK4 and GRK5. These studies demonstrate, for the first time, that BBR exerts renoprotection in high-fat diet and STZ-induced DN rats by modulating the proteins expression of GRKs in G protein- AC-cAMP signaling pathway.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GRK2HumanDiabetic Nephropathies treatmentISOGrk2 (Rattus norvegicus) RGD 
GRK3HumanDiabetic Nephropathies treatmentISOGrk3 (Rattus norvegicus) RGD 
GRK4HumanDiabetic Nephropathies  ISOGrk4 (Rattus norvegicus)protein:decreased expression:cortex of kidney (rat)RGD 
GRK6HumanDiabetic Nephropathies treatmentISOGrk6 (Rattus norvegicus) RGD 
Grk2RatDiabetic Nephropathies treatmentIEP  RGD 
Grk2MouseDiabetic Nephropathies treatmentISOGrk2 (Rattus norvegicus) RGD 
Grk3RatDiabetic Nephropathies treatmentIEP  RGD 
Grk3MouseDiabetic Nephropathies treatmentISOGrk3 (Rattus norvegicus) RGD 
Grk4RatDiabetic Nephropathies  IEP protein:decreased expression:cortex of kidney (rat)RGD 
Grk4MouseDiabetic Nephropathies  ISOGrk4 (Rattus norvegicus)protein:decreased expression:cortex of kidney (rat)RGD 
Grk6RatDiabetic Nephropathies treatmentIEP  RGD 
Grk6MouseDiabetic Nephropathies treatmentISOGrk6 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Grk2  (G protein-coupled receptor kinase 2)
Grk3  (G protein-coupled receptor kinase 3)
Grk4  (G protein-coupled receptor kinase 4)
Grk6  (G protein-coupled receptor kinase 6)

Genes (Mus musculus)
Grk2  (G protein-coupled receptor kinase 2)
Grk3  (G protein-coupled receptor kinase 3)
Grk4  (G protein-coupled receptor kinase 4)
Grk6  (G protein-coupled receptor kinase 6)

Genes (Homo sapiens)
GRK2  (G protein-coupled receptor kinase 2)
GRK3  (G protein-coupled receptor kinase 3)
GRK4  (G protein-coupled receptor kinase 4)
GRK6  (G protein-coupled receptor kinase 6)


Additional Information