RGD Reference Report - Nuclear translocation of calpain-2 regulates propensity toward apoptosis in cardiomyocytes of tail-suspended rats. - Rat Genome Database

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Nuclear translocation of calpain-2 regulates propensity toward apoptosis in cardiomyocytes of tail-suspended rats.

Authors: Chang, Hui  Zhang, Lin  Xu, Peng-Tao  Li, Quan  Sheng, Juan-Juan  Wang, Yun-Ying  Chen, Yan  Zhang, Lan-Ning  Yu, Zhi-Bin 
Citation: Chang H, etal., J Cell Biochem. 2011 Feb;112(2):571-80. doi: 10.1002/jcb.22947.
RGD ID: 13792651
Pubmed: PMID:21268078   (View Abstract at PubMed)
DOI: DOI:10.1002/jcb.22947   (Journal Full-text)

The compensatory increase in catecholamine release does not reverse orthostatic intolerance after returning from a long-term spaceflight, but it is unclear whether high dose of catecholamine induces cardiac damage. The tail-suspended rat model was used to simulate the effects of weightlessness on the heart. Apoptotic rates in the left ventricular myocardium did not increase in 4-week of tail-suspended rats compared with the synchronous control. On the contrary, isoproterenol (intraperitoneal injection) and 1-day recovery from the 4-week tail-suspension increased apoptotic rates in the myocardium. Propranolol and PD150606 inhibited cardiomyocyte apoptosis in the recovery group. PD150606 and calpain-2 knockdown also blocked isoproterenol-induced cardiomyocyte apoptosis in tail-suspended rats. The activity and nuclear translocation of calpain-2 increased, but the expression of calpain-1, calpain-2, and calpastatin was unchanged in the myocardium of tail-suspended rats. The Ser-16-phosphorylated phospholamban of the nuclear envelope was higher in tail-suspended rats than in the control rats under isoproterenol stimulation. Isoproterenol treatment also induced a large intranuclear Ca(2+) transient of cardiomyocytes in tail-suspended rats. These results suggest that high-dose isoproterenol phosphorylates phospholamban of the nuclear envelope and increases intranuclear Ca(2+) transient. Larger intranuclear Ca(2+) further activates nuclear calpain-2 and hence induces cardiomyocyte apoptosis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Capn2Ratpositive regulation of cardiac muscle cell apoptotic process  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Capn2  (calpain 2)


Additional Information