RGD Reference Report - Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor beta1 subunit. - Rat Genome Database

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Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor beta1 subunit.

Authors: Huntsman, Molly M  Huguenard, John R 
Citation: Huntsman MM and Huguenard JR, J Physiol. 2006 Apr 15;572(Pt 2):459-75. doi: 10.1113/jphysiol.2006.106617. Epub 2006 Feb 9.
RGD ID: 13792528
Pubmed: PMID:16469775   (View Abstract at PubMed)
PMCID: PMC1779681   (View Article at PubMed Central)
DOI: DOI:10.1113/jphysiol.2006.106617   (Journal Full-text)

Synchrony within the thalamocortical system is regulated in part by intranuclear synaptic inhibition within the reticular nucleus (RTN). Inhibitory postsynaptic currents (IPSCs) in RTN neurons are largely characterized by slow decay kinetics that result in powerful and prolonged suppression of spikes. Here we show that some individual RTN neurons are characterized by highly variable mixtures of fast, slow and mixed IPSCs. Heterogeneity arose largely through differences in the contribution of an initial decay component (tau(D) approximately 10 ms) which was insensitive to loreclezole, suggesting involvement of the GABA(A) receptor beta(1) subunit. Single-cell RT-PCR revealed the presence of beta(1) subunit mRNA only in those neurons whose IPSCs were dominated by a rapid and prominent initial decay phase. These data show that brief, beta(1)-dependent, loreclezole-insensitive IPSCs are present in a subpopulation of RTN neurons, and suggest that striking differences in IPSC heterogeneity within single neurons can result from of the presence or absence of a single GABA(A) receptor subunit.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gabrb1RatGABA-ergic synapse is_active_inEXP PMID:16469775SynGO 
Gabrb1RatGABA-ergic synapse is_active_inIDA PMID:16469775SynGO 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Gabrb1  (gamma-aminobutyric acid type A receptor subunit beta1)


Additional Information