RGD Reference Report - Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti. - Rat Genome Database

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Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti.

Authors: McLean, Will J  Smith, K Anne  Glowatzki, Elisabeth  Pyott, Sonja J 
Citation: McLean WJ, etal., J Assoc Res Otolaryngol. 2009 Mar;10(1):37-49. doi: 10.1007/s10162-008-0152-9. Epub 2008 Dec 12.
RGD ID: 13702444
Pubmed: PMID:19082858   (View Abstract at PubMed)
PMCID: PMC2644389   (View Article at PubMed Central)
DOI: DOI:10.1007/s10162-008-0152-9   (Journal Full-text)

Processing of sound in the cochlea involves both afferent and efferent innervation. The Na,K-ATPase (NKA) is essential for cells that maintain hyperpolarized membrane potentials and sodium and potassium concentration gradients. Heterogeneity of NKA subunit expression is one mechanism that tailors physiology to particular cellular demands. Therefore, to provide insight into molecular differences that distinguish the various innervation pathways in the cochlea, we performed a variety of double labeling experiments with antibodies against three of the alpha isoforms of the NKA (NKA alpha 1-3) and markers identifying particular subsets of neurons or supporting cells in whole mount preparations of the organ of Corti and spiral ganglion. We found that the NKA alpha 3 is abundantly expressed within the membranes of the spiral ganglion somata, the type I afferent terminals contacting the inner hair cells, and the medial efferent terminals contacting the outer hair cells. We also found expression of the NKA alpha 1 in the supporting cells that neighbor the inner hair cells and express the glutamate transporter GLAST. These findings suggest that both the NKA alpha 1 and NKA alpha 3 are poised to play an essential role in the regulation of the type I afferent synapses, the medial efferent synapses, and also glutamate transport from the afferent-inner hair cell synapse.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
postsynapse is_active_inIDA 13702444PMID:19082858SynGO 
presynaptic membrane is_active_inIDA 13702444PMID:19082858SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Atp1a3  (ATPase Na+/K+ transporting subunit alpha 3)


Additional Information