RGD Reference Report - Proteomics analysis of rat brain postsynaptic density. Implications of the diverse protein functional groups for the integration of synaptic physiology. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Proteomics analysis of rat brain postsynaptic density. Implications of the diverse protein functional groups for the integration of synaptic physiology.

Authors: Li, Ka Wan  Hornshaw, Martin P  Van Der Schors, Roel C  Watson, Rod  Tate, Stephen  Casetta, Bruno  Jimenez, Connie R  Gouwenberg, Yvonne  Gundelfinger, Eckart D  Smalla, Karl-Heinz  Smit, August B 
Citation: Li KW, etal., J Biol Chem. 2004 Jan 9;279(2):987-1002. doi: 10.1074/jbc.M303116200. Epub 2003 Oct 7.
RGD ID: 13702402
Pubmed: PMID:14532281   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M303116200   (Journal Full-text)

The postsynaptic density contains multiple protein complexes that together relay the presynaptic neurotransmitter input to the activation of the postsynaptic neuron. In the present study we took two independent proteome approaches for the characterization of the protein complement of the postsynaptic density, namely 1) two-dimensional gel electrophoresis separation of proteins in conjunction with mass spectrometry to identify the tryptic peptides of the protein spots and 2) isolation of the trypsin-digested sample that was labeled with isotope-coded affinity tag, followed by liquid chromatography-tandem mass spectrometry for the partial separation and identification of the peptides, respectively. Functional grouping of the identified proteins indicates that the postsynaptic density is a structurally and functionally complex organelle that may be involved in a broad range of synaptic activities. These proteins include the receptors and ion channels for glutamate neurotransmission, proteins for maintenance and modulation of synaptic architecture, sorting and trafficking of membrane proteins, generation of anaerobic energy, scaffolding and signaling, local protein synthesis, and correct protein folding and breakdown of synaptic proteins. Together, these results imply that the postsynaptic density may have the ability to function (semi-) autonomously and may direct various cellular functions in order to integrate synaptic physiology.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
translation at postsynapse involved_inNAS 13702402PMID:14532281SynGO 
translation at presynapse involved_inNAS 13702402PMID:14532281SynGO 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
postsynapse is_active_inNAS 13702402PMID:14532281SynGO 
postsynaptic density is_active_inEXP 13702402; 13702402; 13702402; 13702402PMID:14532281SynGO 
postsynaptic density is_active_inIDA 13702402; 13702402; 13702402; 13702402PMID:14532281SynGO 
presynapse is_active_inNAS 13702402PMID:14532281SynGO 
ribosome is_active_inNAS 13702402PMID:14532281SynGO 
synapse is_active_inEXP 13702402; 13702402; 13702402PMID:14532281SynGO 
synapse is_active_inIDA 13702402; 13702402; 13702402PMID:14532281SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Eif5a  (eukaryotic translation initiation factor 5A)
Hnrnph1  (heterogeneous nuclear ribonucleoprotein H1)
Hnrnph2  (heterogeneous nuclear ribonucleoprotein H2)
Pcbp1  (poly(rC) binding protein 1)
Pura  (purine rich element binding protein A)
Rplp0  (ribosomal protein lateral stalk subunit P0)
Tufm  (Tu translation elongation factor, mitochondrial)

Objects referenced in this article
Gene AC099137.1 null Rattus norvegicus

Additional Information