RGD Reference Report - Functional and immunocytochemical characterization of the creatine transporter in rat hippocampal neurons. - Rat Genome Database

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Functional and immunocytochemical characterization of the creatine transporter in rat hippocampal neurons.

Authors: Dodd, Joanna R  Birch, Nigel P  Waldvogel, Henry J  Christie, David L 
Citation: Dodd JR, etal., J Neurochem. 2010 Nov;115(3):684-93. doi: 10.1111/j.1471-4159.2010.06957.x. Epub 2010 Sep 27.
RGD ID: 13702397
Pubmed: PMID:20731764   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2010.06957.x   (Journal Full-text)

Creatine uptake by neurons requires a specific creatine transporter (CRT). The purpose of the present work was to investigate the activity and localization of the CRT in primary cultures of hippocampal neurons obtained from 18-day rat embryos. Creatine uptake increased as the neurons differentiated in culture. Immunofluorescence microscopy showed most of the CRT was associated with dendrites, although some CRT was present in axons and axon terminals. Neurons contained high levels of Na(+)-dependent creatine transport activity (K(m) = 45.5 µM; V(max), 1719 pmol creatine/min/mg protein) which was inhibited by competitive inhibitors of the CRT. The IC(50) for guanidinoacetate, a precursor of creatine, was 712 µM, ~ 15-fold higher than the K(m) for creatine. Incubation of neurons with 1 mM creatine resulted in the accumulation of high levels of creatine which affected the V(max) but not the K(m) for creatine transport. The rate of creatine release from neurons increased in the absence of Na(+) showing the importance of the electrochemical gradient for creatine retention. This is the first detailed study of the CRT in neurons and identifies primary cultures of rat hippocampal neurons as a good model for future studies of the CRT in relation to the effects of creatine on neuronal function and viability.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein catabolic process involved_inEXP 13702397PMID:20731764SynGO 
protein catabolic process involved_inIDA 13702397PMID:20731764SynGO 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glutamatergic synapse is_active_inIDA 13702397PMID:20731764SynGO 
postsynaptic membrane is_active_inIDA 13702397PMID:20731764SynGO 
synapse is_active_inEXP 13702397PMID:20731764SynGO 
synapse is_active_inIDA 13702397PMID:20731764SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Slc6a8  (solute carrier family 6 member 8)


Additional Information