RGD Reference Report - Cortactin-binding protein 2 modulates the mobility of cortactin and regulates dendritic spine formation and maintenance. - Rat Genome Database

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Cortactin-binding protein 2 modulates the mobility of cortactin and regulates dendritic spine formation and maintenance.

Authors: Chen, Yi-Kai  Hsueh, Yi-Ping 
Citation: Chen YK and Hsueh YP, J Neurosci. 2012 Jan 18;32(3):1043-55. doi: 10.1523/JNEUROSCI.4405-11.2012.
RGD ID: 13702362
Pubmed: PMID:22262902   (View Abstract at PubMed)
PMCID: PMC6621164   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.4405-11.2012   (Journal Full-text)

Dendritic spines, the actin-rich protrusions emerging from dendrites, are the locations of excitatory synapses in mammalian brains. Many molecules that regulate actin dynamics also influence the morphology and/or density of dendritic spines. Since dendritic spines are neuron-specific subcellular structures, neuron-specific proteins or signals are expected to control spinogenesis. In this report, we characterize the distribution and function of neuron-predominant cortactin-binding protein 2 (CTTNBP2) in rodents. An analysis of an Expressed Sequence Tag database revealed three splice variants of mouse CTTNBP2: short, long, and intron. Immunoblotting indicated that the short form is the dominant CTTNBP2 variant in the brain. CTTNBP2 proteins were highly concentrated at dendritic spines in cultured rat hippocampal neurons as well as in the mouse brain. Knockdown of CTTNBP2 in neurons reduced the density and size of dendritic spines. Consistent with these morphological changes, the frequencies of miniature EPSCs in CTTNBP2 knockdown neurons were lower than those in control neurons. Cortactin acts downstream of CTTNBP2 in spinogenesis, as the defects caused by CTTNBP2 knockdown were rescued by overexpression of cortactin but not expression of a CTTNBP2 mutant protein lacking the cortactin interaction. Finally, immunofluorescence staining demonstrated that, unlike cortactin, CTTNBP2 stably resided at dendritic spines even after glutamate stimulation. Fluorescence recovery after photobleaching further suggested that CTTNBP2 modulates the mobility of cortactin in neurons. CTTNBP2 may thus help to immobilize cortactin in dendritic spines and control the density of dendritic spines.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glutamatergic synapse is_active_inIDA 13702362PMID:22262902SynGO 
glutamatergic synapse is_active_inIMP 13702362PMID:22262902SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Cttnbp2  (cortactin binding protein 2)


Additional Information