RGD Reference Report - Trans-synaptic adhesion between NGL-3 and LAR regulates the formation of excitatory synapses. - Rat Genome Database

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Trans-synaptic adhesion between NGL-3 and LAR regulates the formation of excitatory synapses.

Authors: Woo, Jooyeon  Kwon, Seok-Kyu  Choi, Seungwon  Kim, Seho  Lee, Jae-Ran  Dunah, Anthone W  Sheng, Morgan  Kim, Eunjoon 
Citation: Woo J, etal., Nat Neurosci. 2009 Apr;12(4):428-37. doi: 10.1038/nn.2279. Epub 2009 Mar 1.
RGD ID: 13702321
Pubmed: (View Article at PubMed) PMID:19252495
DOI: Full-text: DOI:10.1038/nn.2279

Synaptic adhesion molecules regulate multiple steps of synapse formation and maturation. The great diversity of neuronal synapses predicts the presence of a large number of adhesion molecules that control synapse formation through trans-synaptic and heterophilic adhesion. We identified a previously unknown trans-synaptic interaction between netrin-G ligand-3 (NGL-3), a postsynaptic density (PSD) 95-interacting postsynaptic adhesion molecule, and leukocyte common antigen-related (LAR), a receptor protein tyrosine phosphatase. NGL-3 and LAR expressed in heterologous cells induced pre- and postsynaptic differentiation in contacting axons and dendrites of cocultured rat hippocampal neurons, respectively. Neuronal overexpression of NGL-3 increased presynaptic contacts on dendrites of transfected neurons. Direct aggregation of NGL-3 on dendrites induced coclustering of excitatory postsynaptic proteins. Knockdown of NGL-3 reduced the number and function of excitatory synapses. Competitive inhibition by soluble LAR reduced NGL-3-induced presynaptic differentiation. These results suggest that the trans-synaptic adhesion between NGL-3 and LAR regulates excitatory synapse formation in a bidirectional manner.

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Genes (Rattus norvegicus)
Lrrc4b  (leucine rich repeat containing 4B)


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