RGD Reference Report - Cyclin-dependent kinase 6 (CDK6) amplification in human gliomas identified using two-dimensional separation of genomic DNA. - Rat Genome Database

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Cyclin-dependent kinase 6 (CDK6) amplification in human gliomas identified using two-dimensional separation of genomic DNA.

Authors: Costello, J F  Plass, C  Arap, W  Chapman, V M  Held, W A  Berger, M S  Su Huang, H J  Cavenee, W K 
Citation: Costello JF, etal., Cancer Res. 1997 Apr 1;57(7):1250-4.
RGD ID: 13702094
Pubmed: PMID:9102208   (View Abstract at PubMed)

DNA amplification is a common mechanism invoked by many human tumors to elicit overexpression of genes whose products are involved in drug resistance or cell proliferation. Although amplified regions in tumor DNA may exceed several megabases in size, segments of amplicons with a high probability of containing gene sequences may be amenable to detection by restriction landmark genomic scanning (RLGS), a high-resolution DNA analysis that separates labeled NotI fragments in two dimensions. Here, we tested this by applying RLGS to matched samples of glioma and normal brain DNA and found tumor-specific amplification of the gene encoding cyclin-dependent kinase 6 (CDK6), an observation not previously reported in human tumors. The CDK6 gene has been localized to chromosome 7q21-22, but in the gliomas studied here, it was not coamplified with either the syntenic MET (7q31) or epidermal growth factor receptor (7p11-p12) genes, suggesting that this may be part of a novel amplicon in gliomas. We then corroborated this finding by identifying both amplification-associated and amplification-independent increases in CDK6 protein levels in gliomas relative to matched normal brain samples. These data implicate the CDK6 gene in genomic amplification and illustrate the potential of RLGS for the more general identification and cloning of novel genes that are amplified in human cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CDK6Humanhigh grade glioma  IAGP DNA:amplification:RGD 
Cdk6Rathigh grade glioma  ISOCDK6 (Homo sapiens)DNA:amplification:RGD 
Cdk6Mousehigh grade glioma  ISOCDK6 (Homo sapiens)DNA:amplification:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cdk6  (cyclin-dependent kinase 6)

Genes (Mus musculus)
Cdk6  (cyclin dependent kinase 6)

Genes (Homo sapiens)
CDK6  (cyclin dependent kinase 6)


Additional Information