RGD Reference Report - Camk2b protects neurons from homocysteine-induced apoptosis with the involvement of HIF-1a signal pathway. - Rat Genome Database

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Camk2b protects neurons from homocysteine-induced apoptosis with the involvement of HIF-1a signal pathway.

Authors: Fang, Min  Feng, Chao  Zhao, Yan-Xin  Liu, Xue-Yuan 
Citation: Fang M, etal., Int J Clin Exp Med. 2014 Jul 15;7(7):1659-68. eCollection 2014.
RGD ID: 13681928
Pubmed: PMID:25126162   (View Abstract at PubMed)
PMCID: PMC4132126   (View Article at PubMed Central)

In our previous study using iTRAQ technique we found that the level of calmodulin-dependent protein kinase 2b (Camk2b) was lower in rats with hyperhomocysteinemia. We presumed that Camk2b might be involved in homocysteine-induced apoptosis and tried to explore its role in this study through the transfection with Camk2b gene. Results showed that neurons of HHcy group had lower activity measured by MTT, higher percentage of apoptotic neurons, lower expression levels of Camk2b mRNA and protein than those in normal group. Neurons with overexpression of Camk2b (Camk2b group) had lower percentage of apoptosis and higher activity than those in control group. After exposure to 2-Methoxyestradiol, the activity of neurons with overexpression of Camk2b was suppressed with more apoptotic cells observed. The expressions of BCL2, eNOS, EP300 and EPO were all elevated at both mRNA and protein levels in neurons of CamK2b group compared with other three groups. Thus, Camk2b protects neurons from Homocysteine-induced apoptosis with the involvement of HIF-1α signal pathway.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to homocysteine  IEP 13681928 RGD 
hippocampal neuron apoptotic process  IEP 13681928 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Camk2b  (calcium/calmodulin-dependent protein kinase II beta)


Additional Information