RGD Reference Report - ZAP is a CRM1-dependent nucleocytoplasmic shuttling protein. - Rat Genome Database

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ZAP is a CRM1-dependent nucleocytoplasmic shuttling protein.

Authors: Liu, Lixin  Chen, Guifang  Ji, Xin  Gao, Guangxia 
Citation: Liu L, etal., Biochem Biophys Res Commun. 2004 Aug 27;321(3):517-23. doi: 10.1016/j.bbrc.2004.06.174.
RGD ID: 13673890
Pubmed: PMID:15358138   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2004.06.174   (Journal Full-text)

The zinc finger antiviral protein (ZAP) is a recently isolated host antiviral factor. It specifically inhibits the replication of Moloney murine leukemia virus (MMLV) and Sindbis virus (SIN) by preventing the accumulation of viral RNA in the cytoplasm. In this report, we demonstrate that ZAP is predominantly localized in the cytoplasm at steady state but shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Two nuclear localization sequences (NLS) and one nuclear export sequence (NES) were identified. One NLS was mapped to amino acids 68-RARVCRRK-75 and the other mapped to a region including amino acids K405 and K406. The NES was mapped to amino acids 284-LEDVSVDV-291. These findings help to understand why ZAP specifically prevents the accumulation of viral RNA in the cytoplasm. These findings also suggest possible functions of ZAP in the nucleus.

Objects referenced in this article
Gene Zc3hav1 zinc finger CCCH-type containing, antiviral 1 Rattus norvegicus

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