RGD Reference Report - FBP1 and p27kip1 expression after sciatic nerve injury: implications for Schwann cells proliferation and differentiation. - Rat Genome Database

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FBP1 and p27kip1 expression after sciatic nerve injury: implications for Schwann cells proliferation and differentiation.

Authors: Yao, Li  Cao, Jianhua  Sun, Huiqing  Guo, Aisong  Li, Aihong  Ben, Zhiyun  Zhang, Haiyan  Wang, Xinxiu  Ding, Zongmei  Yang, Xiaojing  Huang, Xianting  Ji, Yuhong  Zhou, Zhengming 
Citation: Yao L, etal., J Cell Biochem. 2014 Jan;115(1):130-40. doi: 10.1002/jcb.24640.
RGD ID: 13673880
Pubmed: (View Article at PubMed) PMID:23939805
DOI: Full-text: DOI:10.1002/jcb.24640

Far Upstream Element (FUSE) Binding Protein 1 (FBP1), first identified as a single-stranded DNA (ssDNA) binding protein that binds to the FUSE, could modulate c-myc mRNA levels and also has been shown to regulate tumor cell proliferation and replication of virus. Typically, FBP1 could active the translation of p27kip1 (p27) and participate in tumor growth. However, the expression and roles of FBP1 in peripheral system lesions and repair are still unknown. In our study, we found that FBP1 protein levels was relatively higher in the normal sciatic nerves, significantly decreased and reached a minimal level at Day 3, and then returned to the normal level at 4 weeks. Spatially, we observed that FBP1 had a major colocation in Schwann cells and FBP1 was connected with Ki-67 and Oct-6. In vitro, we detected the decreased level of FBP1 and p27 in the TNF-α-induced Schwann cells proliferation model, while increased expression in cAMP-induced Schwann cells differentiation system. Specially, FBP1-specific siRNA-transfected SCs did not show fine and longer morphological change after cAMP treatment and had a decreased motility compared with normal. At 3 days after cAMP treatment and SC/neuron co-cultures, p27 was transported to cytoplasm to form CDK4/6-p27 to participate in SCs differentiation. In conclusion, we speculated that FBP1 and p27 were involved in SCs proliferation and the following differentiation in the sciatic nerve after crush by transporting p27 from nucleus to cytoplasm.

Annotation

Disease Annotations    
sciatic neuropathy  (IEP,ISO)

Gene Ontology Annotations    

Biological Process

Cellular Component
nucleus  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Fubp1  (far upstream element binding protein 1)

Genes (Mus musculus)
Fubp1  (far upstream element (FUSE) binding protein 1)

Genes (Homo sapiens)
FUBP1  (far upstream element binding protein 1)


Additional Information