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CD36 is indispensable for thermogenesis under conditions of fasting and cold stress.

Authors: Putri, Mirasari  Syamsunarno, Mas Rizky A A  Iso, Tatsuya  Yamaguchi, Aiko  Hanaoka, Hirofumi  Sunaga, Hiroaki  Koitabashi, Norimichi  Matsui, Hiroki  Yamazaki, Chiho  Kameo, Satomi  Tsushima, Yoshito  Yokoyama, Tomoyuki  Koyama, Hiroshi  Abumrad, Nada A  Kurabayashi, Masahiko 
Citation: Putri M, etal., Biochem Biophys Res Commun. 2015 Feb 20;457(4):520-5. doi: 10.1016/j.bbrc.2014.12.124. Epub 2015 Jan 13.
Pubmed: (View Article at PubMed) PMID:25596128
DOI: Full-text: DOI:10.1016/j.bbrc.2014.12.124

Hypothermia can occur during fasting when thermoregulatory mechanisms, involving fatty acid (FA) utilization, are disturbed. CD36/FA translocase is a membrane protein which facilitates membrane transport of long-chain FA in the FA consuming heart, skeletal muscle (SkM) and adipose tissues. It also accelerates uptake of triglyceride-rich lipoprotein by brown adipose tissue (BAT) in a cold environment. In mice deficient for CD36 (CD36(-/-) mice), FA uptake is markedly reduced with a compensatory increase in glucose uptake in the heart and SkM, resulting in lower levels of blood glucose especially during fasting. However, the role of CD36 in thermogenic activity during fasting remains to be determined. In fasted CD36(-/-) mice, body temperature drastically decreased shortly after cold exposure. The hypothermia was accompanied by a marked reduction in blood glucose and in stores of triacylglycerols in BAT and of glycogen in glycolytic SkM. Biodistribution analysis using the FA analogue (125)I-BMIPP and the glucose analogue (18)F-FDG revealed that uptake of FA and glucose was severely impaired in BAT and glycolytic SkM in cold-exposed CD36(-/-) mice. Further, induction of the genes of thermogenesis in BAT was blunted in fasted CD36(-/-) mice after cold exposure. These findings strongly suggest that CD36(-/-) mice exhibit pronounced hypothermia after fasting due to depletion of energy storage in BAT and glycolytic SkM and to reduced supply of energy substrates to these tissues. Our study underscores the importance of CD36 for nutrient homeostasis to survive potentially life-threatening challenges, such as cold and starvation.

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RGD Object Information
RGD ID: 13673833
Created: 2018-06-23
Species: All species
Last Modified: 2018-06-23
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.