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UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction.

Authors: Kazak, Lawrence  Chouchani, Edward T  Stavrovskaya, Irina G  Lu, Gina Z  Jedrychowski, Mark P  Egan, Daniel F  Kumari, Manju  Kong, Xingxing  Erickson, Brian K  Szpyt, John  Rosen, Evan D  Murphy, Michael P  Kristal, Bruce S  Gygi, Steven P  Spiegelman, Bruce M 
Citation: Kazak L, etal., Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):7981-7986. doi: 10.1073/pnas.1705406114. Epub 2017 Jun 19.
Pubmed: (View Article at PubMed) PMID:28630339
DOI: Full-text: DOI:10.1073/pnas.1705406114

Brown adipose tissue (BAT) mitochondria exhibit high oxidative capacity and abundant expression of both electron transport chain components and uncoupling protein 1 (UCP1). UCP1 dissipates the mitochondrial proton motive force (¿p) generated by the respiratory chain and increases thermogenesis. Here we find that in mice genetically lacking UCP1, cold-induced activation of metabolism triggers innate immune signaling and markers of cell death in BAT. Moreover, global proteomic analysis reveals that this cascade induced by UCP1 deletion is associated with a dramatic reduction in electron transport chain abundance. UCP1-deficient BAT mitochondria exhibit reduced mitochondrial calcium buffering capacity and are highly sensitive to mitochondrial permeability transition induced by reactive oxygen species (ROS) and calcium overload. This dysfunction depends on ROS production by reverse electron transport through mitochondrial complex I, and can be rescued by inhibition of electron transfer through complex I or pharmacologic depletion of ROS levels. Our findings indicate that the interscapular BAT of Ucp1 knockout mice exhibits mitochondrial disruptions that extend well beyond the deletion of UCP1 itself. This finding should be carefully considered when using this mouse model to examine the role of UCP1 in physiology.

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RGD Object Information
RGD ID: 13673799
Created: 2018-06-23
Species: All species
Last Modified: 2018-06-23
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.