RGD Reference Report - Autocrine effect of vascular endothelial growth factor-A is essential for mitochondrial function in brown adipocytes. - Rat Genome Database

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Autocrine effect of vascular endothelial growth factor-A is essential for mitochondrial function in brown adipocytes.

Authors: Mahdaviani, Kiana  Chess, David  Wu, Yuanyuan  Shirihai, Orian  Aprahamian, Tamar R 
Citation: Mahdaviani K, etal., Metabolism. 2016 Jan;65(1):26-35. doi: 10.1016/j.metabol.2015.09.012. Epub 2015 Sep 25.
RGD ID: 13673738
Pubmed: PMID:26683794   (View Abstract at PubMed)
PMCID: PMC4684900   (View Article at PubMed Central)
DOI: DOI:10.1016/j.metabol.2015.09.012   (Journal Full-text)


OBJECTIVE: The obesity epidemic in the United States, as well as the accompanying condition of type 2 diabetes, puts a majority of the population at an increased risk of developing cardiovascular diseases including coronary artery disease, stroke, and myocardial infarction. In contrast to white adipose tissue (WAT), brown adipose tissue (BAT) is well vascularized, rich in mitochondria, and highly oxidative. While it is known that the angiogenic factor VEGF-A is required for brown adipocyte development, the functional consequences and exact mechanism remain to be elucidated. Here, we show that VEGF-A plays an essential autocrine role in the function of BAT.
MATERIALS AND METHODS: Mouse models were generated with an adipose-specific and macrophage-specific ablation of VEGF-A. Adipose tissue characteristics and thermogenic response were analyzed in vivo, and mitochondrial morphology and oxidative respiration were analyzed in vitro to assess effects of endogenous VEGF-A ablation.
RESULTS: VEGF-A expression levels are highest in adipocyte precursors compared to immune or endothelial cell populations within both WAT and BAT. Loss of VEGF-A in adipocytes, but not macrophages, results in decreased adipose tissue vascularization, with remarkably diminished thermogenic capacity in vivo. Complete ablation of endogenous VEGF-A decreases oxidative capacity of mitochondria in brown adipocytes. Further, acute ablation of VEGF-A in brown adipocytes in vitro impairs mitochondrial respiration, despite similar mitochondrial mass compared to controls.
CONCLUSION: These data demonstrate that VEGF-A serves to orchestrate the acquisition of thermogenic capacity of brown adipocytes through mitochondrial function in conjunction with the recruitment of blood vessels.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cold-induced thermogenesis involved_inISSUniProtKB:Q0073113673738PMID:26683794YuBioLab 

Objects Annotated

Genes (Rattus norvegicus)
Vegfa  (vascular endothelial growth factor A)


Additional Information