RGD Reference Report - Integrin ß1 Increases Stem Cell Survival and Cardiac Function after Myocardial Infarction. - Rat Genome Database

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Integrin ß1 Increases Stem Cell Survival and Cardiac Function after Myocardial Infarction.

Authors: Li, Lili  Guan, Qifan  Dai, Shuling  Wei, Wen  Zhang, Yao 
Citation: Li L, etal., Front Pharmacol. 2017 Mar 17;8:135. doi: 10.3389/fphar.2017.00135. eCollection 2017.
RGD ID: 13602094
Pubmed: PMID:28367125   (View Abstract at PubMed)
PMCID: PMC5355448   (View Article at PubMed Central)
DOI: DOI:10.3389/fphar.2017.00135   (Journal Full-text)

Bone mesenchymal stem cells (BMSCs) transplantation is a promising therapeutic approach for myocardial infarction (MI), but its application is limited by poor viability of BMSCs. In this study, we aimed to improve the survival of BMSCs by lentivirus vector mediated overexpression of integrin ß1. In vitro study showed that integrin ß1 overexpression could facilitate the proliferation of BMSCs under oxygen glucose deprivation condition and regulated the expression of Caspase-3, Bax, Bcl-2, FAK, and ILK in BMSCs. Next, MI was induced in rat model and Igtb1BMSCs, NullBMSCs, or NatBMSCs were transplanted by intramyocardial injection. One week later, the survival of BMSCs was higher in Itgb1 BMSCs group than in other groups. Four weeks after transplantation, heart function was significantly improved in Igtb1BMSCs group compared to other groups. The expression levels of Caspase-3 and Bax were decreased while the expression levels of Bcl-2, FAK, ILK, and VEGF were increased in the cardiomyocytes of Igtb1BMSCs group compared to other groups. In conclusion, integrin ß1 overexpression could increase the survival of BMSCs and improve the efficacy of transplanted BMSCs for MI treatment. The beneficial effects may be mediated by inhibiting the apoptosis of both transplanted BMSCs and cardiomyocytes through adhesion-mediated cell survival signaling.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
myocardial infarction treatmentISOItgb1 (Rattus norvegicus)13602094; 13602094 RGD 
myocardial infarction treatmentIMP 13602094 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of apoptotic process  IDA 13602094 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Itgb1  (integrin subunit beta 1)

Genes (Mus musculus)
Itgb1  (integrin beta 1 (fibronectin receptor beta))

Genes (Homo sapiens)
ITGB1  (integrin subunit beta 1)


Additional Information