RGD Reference Report - The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity. - Rat Genome Database

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The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.

Authors: Hermann, A  Schmitt, S  Jeltsch, A 
Citation: Hermann A, etal., J Biol Chem 2003 Aug 22;278(34):31717-21. Epub 2003 Jun 6.
RGD ID: 1359088
Pubmed: PMID:12794065   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M305448200   (Journal Full-text)

The human Dnmt2 protein is one member of a protein family conserved from Schizosaccharomyces pombe and Drosophila melanogaster to Mus musculus and Homo sapiens. It contains all of the amino acid motifs characteristic for DNA-(Cytosine-C5) methyltransferases, and its structure is very similar to prokaryotic DNA methyltransferases. Nevertheless, so far all attempts to detect catalytic activity of this protein have failed. We show here by two independent assay systems that the purified Dnmt2 protein has weak DNA methyltransferase activity. Methylation was observed at CG sites in a loose ttnCGga(g/a) consensus sequence, suggesting that Dnmt2 has a more specialized role than other mammalian DNA methyltransferases.

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Trdmt1Rathomocysteine metabolic pathway   ISO RGD 
Trdmt1Ratmethionine cycle/metabolic pathway   ISO RGD 
Objects Annotated

Genes (Rattus norvegicus)
Trdmt1  (tRNA aspartic acid methyltransferase 1)

Additional Information