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Estimate of the genetic divergence between inbred Dahl salt-sensitive and salt-resistant rats.

Authors: Ginn, DI  Baptista, CA  Alam, KY  Deng, AY  Cicila, GT  Margolius, HS  Rapp, JP 
Citation: Ginn DI, etal., J Hypertens 1993 May;11(5):477-81.
Pubmed: (View Article at PubMed) PMID:8099934

OBJECTIVE: The genetic divergence of inbred Dahl salt-sensitive (SS/Jr) rats from inbred Dahl salt-resistant (SR/Jr) rats and various other inbred strains was measured. DESIGN: Structural differences in DNA between strains were evaluated. METHODS: Genetic variants were sought (1) by restriction fragment length polymorphism (RFLP) analysis, using 19 DNA probes, (2) by the polymerase chain reaction around microsatellites and (3) by DNA sequencing. RESULTS: It was estimated that 1 in 1532 bases of DNA were different between the SS/Jr and SR/Jr strains. In comparing SS/Jr and SR/Jr rats, it was also observed that one DNA probe in 10 will yield multiple RFLP, presumably as the result of large insertion/deletion events. A comparison of SS/Jr rats with seven other inbred strains showed that the percentage of loci that carry alleles different from SS/Jr rats varies from about 23% for Albino Surgery rats to 71% for Brown Norway rats. CONCLUSIONS: Although the SR/Jr strain is an appropriate contrasting strain for the genetic analysis of hypertension in SS/Jr rats, a genetic analysis involving crosses of SS/Jr rats and unrelated inbred strains is also likely to be useful in identifying genes that cosegregate with blood pressure because more informative genetic markers will be available than in a cross of SS/Jr with SR/Jr rats.

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RGD ID: 1358976
Created: 2005-07-19
Species: All species
Last Modified: 2005-07-19
Status: ACTIVE



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