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Transcription of sequences upstream of the rat prolactin gene suggests the existence of a second promoter.

Authors: Shull, JD 
Citation: Shull JD Mol Cell Endocrinol 1991 Nov;82(1):R17-23.
Pubmed: (View Article at PubMed) PMID:1722177

The transcription of the rat prolactin gene domain has been examined using a modified Southern blot procedure. Cloned genomic DNAs were resolved by electrophoresis in agarose, transferred to nitrocellulose, and probed with radiolabeled RNA that had been synthesized in vitro by nuclei isolated from pituitary tumor cells. Data presented in this paper illustrate that single copy genomic sequences located within 7.3 kb upstream of exon 1 are transcribed. Single copy or low copy number DNA sequences that reside greater than 7.3 kb upstream of exon 1, or downstream of exon 5 were not transcribed at detectable levels. These data suggest that a second promoter may exist upstream of the rat prolactin gene and that this second promoter may be active in pituitary cells.


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RGD Object Information
RGD ID: 1358951
Created: 2005-07-16
Species: All species
Last Modified: 2005-07-16
Status: ACTIVE


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