RGD Reference Report - Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat. - Rat Genome Database

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Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat.

Authors: Muramatsu, Y  Yamada, T  Taniguchi, Y  Ogino, T  Kose, H  Matsumoto, K  Sasaki, Y 
Citation: Muramatsu Y, etal., Biochem Biophys Res Commun 2005 Jun 17;331(4):1270-6.
RGD ID: 1358859
Pubmed: (View Article at PubMed) PMID:15883013
DOI: Full-text: DOI:10.1016/j.bbrc.2005.04.040

The Otsuka Long-Evans Tokushima Fatty (OLETF) rat exhibits polygenic obesity, and one of quantitative trait loci (QTLs) responsible for a susceptibility to obesity in the OLETF, Nidd6/of, has been mapped to the approximately 10-cM genomic region between D1Rat166 and D1Rat90 on chromosome 1 in (OLETF x normal) F2 intercross. In this study, we have attempted to identify the causal gene for the Nidd6/of QTL. A Nidd6/of congenic strain, constructed by introgressing the OLETF allele on the mapped Nidd6/of region in the normal F344 rat strain, confirmed the existence of the Nidd6/of as obesity QTL. The Nidd6/of region was refined to a approximately 2.3-cM genomic region between D1Rat225 and D1Rat90, using informative recombinants selected from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. Among 46 genes located within the approximately 2.3-cM region, pancreatic lipase gene, Pnlip, was regarded as the most prominent and physiologically relevant positional candidate for the Nidd6/of QTL. We found that Pnlip possesses an OLETF allele-specific increase of mRNA levels in the pancreas, and that the OLETF allele is longer in variable number of tandem repeat (VNTR) within the 5'-flanking region than normal alleles. We further showed that the Nidd6/of QTL completely cosegregates with Pnlip VNTR in the informative recombinants from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. These results suggest that Pnlip is possible candidate for the Nidd6/of QTL.



Disease Annotations    
obesity  (IAGP,ISO)

Phenotype Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Pnlip  (pancreatic lipase)

Genes (Mus musculus)
Pnlip  (pancreatic lipase)

Genes (Homo sapiens)
PNLIP  (pancreatic lipase)

QTLs
Bw54  (Body weight QTL 54)

Strains
F344.OLETF-(D1Rat166-D1Rat90)/Tj  (NA)
OLETF/Got  (Otsuka Long Evans Tokushima)

Objects referenced in this article
QTL Bw115 Body weight QTL 115 Rattus norvegicus
QTL Niddm24 Non-insulin dependent diabetes mellitus QTL 24 Rattus norvegicus

Additional Information