RGD Reference Report - Regulation of rat pancreatic CCKB receptor and somatostatin expression by insulin. - Rat Genome Database

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Regulation of rat pancreatic CCKB receptor and somatostatin expression by insulin.

Authors: Julien, S  Laine, J  Morisset, J 
Citation: Julien S, etal., Diabetes 2004 Jun;53(6):1526-34.
RGD ID: 1358452
Pubmed: PMID:15161757   (View Abstract at PubMed)

The cholecystokinin B receptor (CCK(B)R) is localized on pancreatic endocrine somatostatin delta-cells. Pancreatic somatostatin content was increased in diabetic rats. The mechanisms involved in this phenomenon are unknown, and we believe insulin is involved. In this study, four groups of rats were used: controls, streptozotocin-induced diabetic, streptozotocin-induced diabetic with insulin, and streptozotocin-induced diabetic with insulin and its cessation. Rats were killed after 7-28 days of treatment for diabetes, and somatostatin mRNA expression and pancreatic somatostatin content, CCK(B)R mRNA and protein expression evaluation in total pancreas and purified islets, and the cellular localization of somatostatin and CCK(B)R in islets was measured. Data indicate that diabetes is established after 7 days, is controlled by insulin, and reappears after treatment cessation. Pancreatic somatostatin mRNA expression and somatostatin content were increased during diabetes, normalized during insulin treatment, and reaugmented after treatment cessation. Gland and islet CCK(B)R mRNA and protein almost disappeared during diabetes; CCK(B) mRNA reappeared in response to insulin, but the protein did not. Confocal microscopy confirmed data obtained on somatostatin and CCK(B)R as established biochemically in the course of the treatments. In conclusion, these data strongly suggest that insulin can negatively control pancreatic somatostatin mRNA and hormone content and positively control CCK(B)R mRNA; the CCK(B)R protein appears to be delayed.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Diabetes Mellitus  ISOCckbr (Rattus norvegicus)1358452; 1358452mRNA and protein:decreased expression:pancreasRGD 
Experimental Diabetes Mellitus  IEP 1358452mRNA and protein:decreased expression:pancreasRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to insulin  IEP 1358452 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cckbr  (cholecystokinin B receptor)

Genes (Mus musculus)
Cckbr  (cholecystokinin B receptor)

Genes (Homo sapiens)
CCKBR  (cholecystokinin B receptor)

Objects referenced in this article
Gene Ins1 insulin 1 Rattus norvegicus
Gene Sst somatostatin Rattus norvegicus

Additional Information