RGD Reference Report - Nucleotide-induced conformational changes of PMP70, an ATP binding cassette transporter on rat liver peroxisomal membranes. - Rat Genome Database

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Nucleotide-induced conformational changes of PMP70, an ATP binding cassette transporter on rat liver peroxisomal membranes.

Authors: Kashiwayama, Y  Morita, M  Kamijo, K  Imanaka, T 
Citation: Kashiwayama Y, etal., Biochem Biophys Res Commun 2002 Mar 15;291(5):1245-51.
RGD ID: 1358265
Pubmed: PMID:11883951   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2002.6588   (Journal Full-text)

Nucleotide-induced conformational changes of the 70-kDa peroxisomal membrane protein (PMP70) were investigated by means of limited-trypsin digestion. Rat liver peroxisomes preincubated with various nucleotides were subsequently digested by trypsin. The digestion products were subjected to immunoblot analysis with an anti-PMP70 antibody that recognizes the carboxyl-terminal 15 amino acids of the protein. PMP70 was initially cleaved in the boundary region between the transmembrane and nucleotide-binding domains and a carboxyl-terminal 30-kDa fragment resulted. The fragment in turn was progressively digested at the helical domain between the Walker A and B motifs. The fragment, however, could be stabilized with MgATP or MgADP. In contrast to MgATP, MgATP-gammaS protected whole PMP70 as well as the fragment. The 30-kDa fragment processed by trypsin was recovered in the post-peroxisomal fraction as a complex with a molecular mass of about 60 kDa irrespective of the presence of MgATP. These results suggest that PMP70 exists as a dimer on the peroxisomal membranes and the binding and hydrolysis of ATP induce conformational changes in PMP70 close to the boundary between the transmembrane and nucleotide binding domains and the helical domain between the Walker A and B motifs.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Abcd3Ratlong-chain fatty acid import into peroxisome  TAS  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Abcd3Ratperoxisomal membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Abcd3RatATPase-coupled transmembrane transporter activity  TAS  RGD 
Abcd3Ratidentical protein binding  IDA homodimerRGD 

Objects Annotated

Genes (Rattus norvegicus)
Abcd3  (ATP binding cassette subfamily D member 3)


Additional Information