RGD Reference Report - Stargazin-related protein ¿7 is associated with signalling endosomes in superior cervical ganglion neurons and modulates neurite outgrowth. - Rat Genome Database

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Stargazin-related protein ¿7 is associated with signalling endosomes in superior cervical ganglion neurons and modulates neurite outgrowth.

Authors: Waithe, Dominic  Ferron, Laurent  Dolphin, Annette C 
Citation: Waithe D, etal., J Cell Sci. 2011 Jun 15;124(Pt 12):2049-57. doi: 10.1242/jcs.084988. Epub 2011 May 24.
RGD ID: 13524563
Pubmed: (View Article at PubMed) PMID:21610096
DOI: Full-text: DOI:10.1242/jcs.084988

The role(s) of the newly discovered stargazin-like γ-subunit proteins remains unclear; although they are now widely accepted to be transmembrane AMPA receptor regulatory proteins (TARPs), rather than Ca²¿ channel subunits, it is possible that they have more general roles in trafficking within neurons. We previously found that γ7 subunit is associated with vesicles when it is expressed in neurons and other cells. Here, we show that γ7 is present mainly in retrogradely transported organelles in sympathetic neurons, where it colocalises with TrkA-YFP, and with the early endosome marker EEA1, suggesting that γ7 localises to signalling endosomes. It was not found to colocalise with markers of the endoplasmic reticulum, mitochondria, lysosomes or late endosomes. Furthermore, knockdown of endogenous γ7 by short hairpin RNA transfection into sympathetic neurons reduced neurite outgrowth. The same was true in the PC12 neuronal cell line, where neurite outgrowth was restored by overexpression of human γ7. These findings open the possibility that γ7 has an essential trafficking role in relation to neurite outgrowth as a component of endosomes involved in neurite extension and growth cone remodelling.



Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Cacng7  (calcium voltage-gated channel auxiliary subunit gamma 7)


Additional Information