RGD Reference Report - MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons. - Rat Genome Database

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MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.

Authors: Gumy, Laura F  Katrukha, Eugene A  Grigoriev, Ilya  Jaarsma, Dick  Kapitein, Lukas C  Akhmanova, Anna  Hoogenraad, Casper C 
Citation: Gumy LF, etal., Neuron. 2017 Apr 19;94(2):347-362.e7. doi: 10.1016/j.neuron.2017.03.046.
RGD ID: 13514087
Pubmed: (View Article at PubMed) PMID:28426968
DOI: Full-text: DOI:10.1016/j.neuron.2017.03.046

Polarized cargo transport is essential for neuronal function. However, the minimal basic components required for selective cargo sorting and distribution in neurons remain elusive. We found that in sensory neurons the axon initial segment is largely absent and that microtubule-associated protein 2 (MAP2) defines the cargo-filtering zone in the proximal axon. Here, MAP2 directs axonal cargo entry by coordinating the activities of molecular motors. We show that distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport. MAP2 inhibits "slow" kinesin-1 motor activity and allows kinesin-3 to drive robust cargo transport from the soma into the axon. In the distal axon, the inhibitory action of MAP2 decreases, leading to regained kinesin-1 activity and vesicle distribution. We propose that selective axonal cargo trafficking requires the MAP2-defined pre-axonal filtering zone and the ability of cargos to switch between distinct kinesin motor activities.

Annotation

Objects Annotated

Genes (Rattus norvegicus)
Ank3  (ankyrin 3)
Kif1a  (kinesin family member 1A)
Kif1b  (kinesin family member 1B)
Kif1c  (kinesin family member 1C)
Kif5a  (kinesin family member 5A)
Kif5b  (kinesin family member 5B)
Map1a  (microtubule-associated protein 1A)
Map1b  (microtubule-associated protein 1B)
Map2  (microtubule-associated protein 2)
Map4  (microtubule-associated protein 4)
Map7  (microtubule-associated protein 7)
Map9  (microtubule-associated protein 9)
Nfasc  (neurofascin)
Sptbn4  (spectrin, beta, non-erythrocytic 4)
Trim46  (tripartite motif-containing 46)


Additional Information