RGD Reference Report - Vitamin E and vitamin C do not reduce insulin sensitivity but inhibit mitochondrial protein expression in exercising obese rats. - Rat Genome Database

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Vitamin E and vitamin C do not reduce insulin sensitivity but inhibit mitochondrial protein expression in exercising obese rats.

Authors: Picklo, Matthew J  Thyfault, John P 
Citation: Picklo MJ and Thyfault JP, Appl Physiol Nutr Metab. 2015 Apr;40(4):343-52. doi: 10.1139/apnm-2014-0302. Epub 2014 Dec 9.
RGD ID: 13513980
Pubmed: PMID:25761734   (View Abstract at PubMed)
PMCID: PMC4713234   (View Article at PubMed Central)
DOI: DOI:10.1139/apnm-2014-0302   (Journal Full-text)

Controversy exists as to whether supplementation with the antioxidants vitamin E and vitamin C blocks adaptation to exercise. Exercise is a first-line means to treat obesity and its complications. While diet-induced obesity alters mitochondrial function and induces insulin resistance (IR), no data exist as to whether supplementation with vitamin E and vitamin C modify responses to exercise in pre-existing obesity. We tested the hypothesis that dietary supplementation with vitamin E (0.4 g α-tocopherol acetate/kg) and vitamin C (0.5 g/kg) blocks exercise-induced improvements on IR and mitochondrial content in obese rats maintained on a high-fat (45% fat energy (en)) diet. Diet-induced obese, sedentary rats had a 2-fold higher homeostasis model assessment of insulin resistance and larger insulin area under the curve following glucose tolerances test than rats fed a low-fat (10% fat en) diet. Exercising (12 weeks at 5 times per week in a motorized wheel) of obese rats normalized IR indices, an effect not modified by vitamin E and vitamin C. Vitamin E and vitamin C supplementation with exercise elevated mtDNA content in adipose and skeletal muscle to a greater extent (20%) than exercise alone in a depot-specific manner. On the other hand, vitamin C and vitamin E decreased exercise-induced increases in mitochondrial protein content for complex I (40%) and nicotinamide nucleotide transhydrogenase (35%) in a muscle-dependent manner. These data indicate that vitamin E and vitamin C supplementation in obese rodents does not modify exercise-induced improvements in insulin sensitivity but that changes in mitochondrial biogenesis and mitochondrial protein expression may be modified by antioxidant supplementation.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NNTHumanobesity  ISONnt (Rattus norvegicus)protein:decreased expression:gastrocnemius and soleusRGD 
NntMouseobesity  ISONnt (Rattus norvegicus)protein:decreased expression:gastrocnemius and soleusRGD 
NntRatobesity  IEP protein:decreased expression:gastrocnemius and soleusRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NntRatresponse to vitamin  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nnt  (nicotinamide nucleotide transhydrogenase)

Genes (Mus musculus)
Nnt  (nicotinamide nucleotide transhydrogenase)

Genes (Homo sapiens)
NNT  (nicotinamide nucleotide transhydrogenase)


Additional Information