RGD Reference Report - Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish. - Rat Genome Database

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Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish.

Authors: Ito, Masahiko  Shikano, Tomohide  Oda, Shoji  Horiguchi, Takashi  Tanimoto, Satomi  Awaji, Takeo  Mitani, Hiroshi  Miyazaki, Shunichi 
Citation: Ito M, etal., Biol Reprod. 2008 Jun;78(6):1081-90. doi: 10.1095/biolreprod.108.067801. Epub 2008 Mar 5.
RGD ID: 13507301
Pubmed: PMID:18322275   (View Abstract at PubMed)
DOI: DOI:10.1095/biolreprod.108.067801   (Journal Full-text)

Mouse phospholipase C, zeta 1 (PLCZ1), a strong candidate of egg-activating sperm factor, induces Ca(2+) oscillations and accumulates into formed pronucleus (PN) when expressed by cRNA injection. These activities were compared among mouse and human PLCZ1, newly cloned rat Plcz1, and medaka fish plcz1. The PLCZ1 proteins of the four species have an approximately homologous sequence of nuclear localization signal. However, the nuclear translocation ability was defective in rat, human, and medaka PLCZ1 expressed in mouse eggs. Rat PLCZ1 could not enter rat PN, whereas mouse PLCZ1 could. Mouse and human PLCZ1 translocated into the nucleus of COS-7 cells transfected with cDNA. There was little medaka PLCZ1 accumulated in the nucleus, and rat PLCZ1 was never located in the nucleus. All PLCZ1 proteins including fish could induce Ca(2+) oscillations in mouse eggs, but the activity was variable in the order of human > mouse > medaka > rat, estimated from minimal RNA concentration to induce Ca(2+) spikes. Ca(2+) oscillations by human PLCZ1 continued far beyond the time of PN formation (T(PN)), whereas those by mouse PLCZ1 ceased slightly before T(PN). High-frequency Ca(2+) spikes by overexpressed rat PLCZ1 stopped far before T(PN), possibly by feedback inhibition. Ca(2+) oscillations by fertilization of rat eggs stopped at T(PN), despite defective nuclear translocation of rat PLCZ1. Thus, PLCZ1 sequestration into PN participates in termination of Ca(2+) oscillations at the interphase of mouse embryos but does not always operate in other mammals, notably in rat embryos.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cytosolic calcium ion concentration involved in egg activation involved_inIDA 13507301PMID:18322275UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoplasm located_inIDA 13507301PMID:18322275UniProt 
pronucleus NOT|located_inIDA 13507301PMID:18322275UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Plcz1  (phospholipase C, zeta 1)


Additional Information