RGD Reference Report - Changes in Expression of Dopamine, Its Receptor, and Transporter in Nucleus Accumbens of Heroin-Addicted Rats with Brain-Derived Neurotrophic Factor (BDNF) Overexpression. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Changes in Expression of Dopamine, Its Receptor, and Transporter in Nucleus Accumbens of Heroin-Addicted Rats with Brain-Derived Neurotrophic Factor (BDNF) Overexpression.

Authors: Li, Yixin  Xia, Baijuan  Li, Rongrong  Yin, Dan  Liang, Wenmei 
Citation: Li Y, etal., Med Sci Monit. 2017 Jun 9;23:2805-2815.
RGD ID: 13506959
Pubmed: PMID:28598964   (View Abstract at PubMed)
PMCID: PMC5473376   (View Article at PubMed Central)

BACKGROUND The aim of this study was to explore how changes in the expression of BDNF in MLDS change the effect of BDNF on dopamine (DA) neurons, which may have therapeutic implications for heroin addiction. MATERIAL AND METHODS We established a rat model of heroin addiction and observed changes in the expression of BDNF, DA, dopamine receptor (DRD), dopamine transporter (DAT), and other relevant pathways in NAc. We also assessed the effect of BDNF overexpression in the NAc, behavioral changes of heroin-conditioned place preference (CPP), and naloxone withdrawal in rats with high levels of BDNF. We established 5 adult male rat groups: heroin addiction, lentivirus transfection, blank virus, sham operation, and control. The PCR gene chip was used to study gene expression changes. BDNF lentivirus transfection was used for BDNF overexpression. A heroin CPP model and a naloxone withdrawal model of rats were established. RESULTS Expression changes were found in 20 of the 84 DA-associated genes in the NAc of heroin-addicted rats. Weight loss and withdrawal symptoms in the lentivirus group for naloxone withdrawal was less than in the blank virus and the sham operation group. These 2 latter groups also showed significant behavioral changes, but such changes were not observed in the BDNF lentivirus group before or after training. DRD3 and DAT increased in the NAc of the lentivirus group. CONCLUSIONS BDNF and DA in the NAc are involved in heroin addiction. BDNF overexpression in NAc reduces withdrawal symptoms and craving behavior for medicine induced by environmental cues for heroin-addicted rats. BDNF participates in the regulation of the dopamine system by acting on DRD3 and DAT.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Drd1  (dopamine receptor D1)
Drd2  (dopamine receptor D2)
Drd3  (dopamine receptor D3)
Slc6a3  (solute carrier family 6 member 3)

Genes (Mus musculus)
Drd1  (dopamine receptor D1)
Drd2  (dopamine receptor D2)
Drd3  (dopamine receptor D3)
Slc6a3  (solute carrier family 6 (neurotransmitter transporter, dopamine), member 3)

Genes (Homo sapiens)
DRD1  (dopamine receptor D1)
DRD2  (dopamine receptor D2)
DRD3  (dopamine receptor D3)
SLC6A3  (solute carrier family 6 member 3)


Additional Information