RGD Reference Report - Changes in the BAG1 expression of Schwann cells after sciatic nerve crush. - Rat Genome Database

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Changes in the BAG1 expression of Schwann cells after sciatic nerve crush.

Authors: Wu, Hao  Liu, Yonghua  Zhou, Yuan  Long, Long  Cheng, Xinghai  Ji, Lei  Weng, Hai  Ding, Tao  Yang, Jiao  Wei, Haixiang  Li, Ming  Huan, Weipeng  Deng, Xiaolong  Wang, Youhua 
Citation: Wu H, etal., J Mol Neurosci. 2013 Mar;49(3):512-22. doi: 10.1007/s12031-012-9910-6. Epub 2012 Oct 30.
RGD ID: 13506903
Pubmed: PMID:23108487   (View Abstract at PubMed)
DOI: DOI:10.1007/s12031-012-9910-6   (Journal Full-text)

Bcl-2-associated athanogene-1 (BAG1), a co-chaperone for Hsp70/Hsc70, is a multifunctional protein, which has been shown to suppress apoptosis and enhance neuronal differentiation. However, the expression and roles of BAG1 in peripheral system lesions and repair are still unknown. In this study, we investigated the dynamic changes in BAG1 expression in an acute sciatic nerve crush model in adult rats. Western blot analysis revealed that BAG1 was expressed in normal sciatic nerves. BAG1 expression increased progressively after sciatic nerve crush, reached a peak 2 weeks post-injury, and then returned to the normal level 4 weeks post-injury. Spatially, we observed that BAG1 was mainly expressed in Schwann cells and that BAG1 expression increased in Schwann cells after injury. In vitro, we found that BAG1 expression increased during the cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation process. BAG1-specific siRNA inhibited cAMP-induced Schwann cell differentiation. In conclusion, we speculated that BAG1 was upregulated in the sciatic nerve after crush, which was associated with Schwann cell differentiation.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lesion of sciatic nerve  ISOBag1 (Rattus norvegicus)13506903; 13506903 RGD 
lesion of sciatic nerve  IEP 13506903 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of Schwann cell differentiation  IMP 13506903 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bag1  (BAG cochaperone 1)

Genes (Mus musculus)
Bag1  (BCL2-associated athanogene 1)

Genes (Homo sapiens)
BAG1  (BAG cochaperone 1)


Additional Information