RGD Reference Report - The human L-type calcium channel Cav1.3 regulates insulin release and polymorphisms in CACNA1D associate with type 2 diabetes. - Rat Genome Database
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The human L-type calcium channel Cav1.3 regulates insulin release and polymorphisms in CACNA1D associate with type 2 diabetes.

Authors: Reinbothe, T M  Alkayyali, S  Ahlqvist, E  Tuomi, T  Isomaa, B  Lyssenko, V  Renström, E 
Citation: Reinbothe TM, etal., Diabetologia. 2013 Feb;56(2):340-9. doi: 10.1007/s00125-012-2758-z. Epub 2012 Nov 15.
RGD ID: 13506727
Pubmed: (View Article at PubMed) PMID:23229155
DOI: Full-text: DOI:10.1007/s00125-012-2758-z


AIMS/HYPOTHESIS: Voltage-gated calcium channels of the L-type have been shown to be essential for rodent pancreatic beta cell function, but data about their presence and regulation in humans are incomplete. We therefore sought to elucidate which L-type channel isoform is functionally important and its association with inherited diabetes-related phenotypes.
METHODS: Beta cells of human islets from cadaver donors were enriched using FACS to study the expression of the genes encoding voltage-gated calcium channel (Cav)1.2 and Cav1.3 by absolute quantitative PCR in whole human and rat islets, as well as in clonal cells. Single-cell exocytosis was monitored as increases in cell capacitance after treatment with small interfering (si)RNA against CACNA1D (which encodes Cav1.3). Three single nucleotide polymorphisms (SNPs) were genotyped in 8,987 non-diabetic and 2,830 type 2 diabetic individuals from Finland and Sweden and analysed for associations with type 2 diabetes and insulin phenotypes.
RESULTS: In FACS-enriched human beta cells, CACNA1D mRNA expression exceeded that of CACNA1C (which encodes Cav1.2) by approximately 60-fold and was decreased in islets from type 2 diabetes patients. The latter coincided with diminished secretion of insulin in vitro. CACNA1D siRNA reduced glucose-stimulated insulin release in INS-1 832/13 cells and exocytosis in human beta cells. Phenotype/genotype associations of three SNPs in the CACNA1D gene revealed an association between the C allele of the SNP rs312480 and reduced mRNA expression, as well as decreased insulin secretion in vivo, whereas both rs312486/G and rs9841978/G were associated with type 2 diabetes.
CONCLUSION/INTERPRETATION: We conclude that the L-type calcium channel Cav1.3 is important in human glucose-induced insulin secretion, and common variants in CACNA1D might contribute to type 2 diabetes.

Annotation

Disease Annotations    
type 2 diabetes mellitus  (IAGP,IEP,ISO)

Gene Ontology Annotations    

Biological Process

Phenotype Annotations    

Human Phenotype
Objects Annotated

Genes (Rattus norvegicus)
Cacna1d  (calcium voltage-gated channel subunit alpha1 D)

Genes (Mus musculus)
Cacna1d  (calcium channel, voltage-dependent, L type, alpha 1D subunit)

Genes (Homo sapiens)
CACNA1D  (calcium voltage-gated channel subunit alpha1 D)


Additional Information