RGD Reference Report - MicroRNA-193-5p modulates angiogenesis through IGF2 in type 2 diabetic cardiomyopathy. - Rat Genome Database

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MicroRNA-193-5p modulates angiogenesis through IGF2 in type 2 diabetic cardiomyopathy.

Authors: Yi, Fan  Shang, Yuqiang  Li, Bing  Dai, Shilin  Wu, Wei  Cheng, Long  Wang, Xiancan 
Citation: Yi F, etal., Biochem Biophys Res Commun. 2017 Sep 30;491(4):876-882. doi: 10.1016/j.bbrc.2017.07.108. Epub 2017 Jul 20.
RGD ID: 13506264
Pubmed: PMID:28735866   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2017.07.108   (Journal Full-text)


BACKGROUND: Patients with diabetic cardiomyopathy are often associated with increasing risk of heart failure. In this work, we used animal model to characterize the angiogenic effect of microRNA-193-5p, miR-193-5p in type 2 diabetic Goto-Kakizaki (GK) rats' myocardial microvascular endothelial cells, MMEC(GK).
METHODS: MiR-193-5p in MMEC(GK) was compared to its expression in Wistar rat MMEC. In MMEC(GK), miR-193-5p was downregulated through viral infection. Its angiogenic effects on MMEC(GK) migration and proliferation were assessed by transwell and MTT assays, respectively. Downstream target of miR-193-5p, insulin growth factor 2 (IGF2), was assessed by dual-luciferase activity, qRT-PCR and western blot assays, respectively. In miR-193-5p-downregulated MMEC(GK), IGF2 was further de-regulated to assess its mechanism in miR-193-5p-downreuglation induced angiogenic regulation.
RESULTS: MiR-193-5p is overexpressed in MMEC(GK). Its downregulation has significantly angiogenic effect by inducing migration and proliferation in MMEC(GK). IGF2 was demonstrated to be directly regulated by miR-193-5p in MMEC(GK). In addition, IGF2 inhibition in miR-193-5p-downregulated MMEC(GK)'s severely hindered cell migration and proliferation.
CONCLUSION: MiR-193-5p is an active angiogenic factor in diabetic cardiomyopathy, possibly through inverse regulation on its downstream IGF2 gene.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of angiogenesis involved_inIGIRNAcentral:URS00004DE9D7_1011613506264PMID:28735866BHF-UCL 
positive regulation of blood vessel endothelial cell migration involved_inIGIRNAcentral:URS00004DE9D7_1011613506264PMID:28735866BHF-UCL 
positive regulation of vascular endothelial cell proliferation involved_inIGIRNAcentral:URS00004DE9D7_1011613506264PMID:28735866BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Igf2  (insulin-like growth factor 2)


Additional Information