RGD Reference Report - Inhibitory role of endophilin 3 in receptor-mediated endocytosis. - Rat Genome Database

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Inhibitory role of endophilin 3 in receptor-mediated endocytosis.

Authors: Sugiura, Hiroko  Iwata, Ken  Matsuoka, Masato  Hayashi, Hiroshi  Takemiya, Takako  Yasuda, Shin  Ichikawa, Masumi  Yamauchi, Takashi  Mehlen, Patrick  Haga, Tatsuya  Yamagata, Kanato 
Citation: Sugiura H, etal., J Biol Chem. 2004 May 28;279(22):23343-8. doi: 10.1074/jbc.M312607200. Epub 2004 Apr 5.
RGD ID: 13504745
Pubmed: (View Article at PubMed) PMID:15066995
DOI: Full-text: DOI:10.1074/jbc.M312607200

Endophilin 1 (Endo1) participates in synaptic vesicle biogenesis through interactions of its Src homology 3 domain with the polyphosphoinositide phosphatase Synaptojanin and the GTPase Dynamin. Endo1 has also been reported to affect endocytosis by converting membrane curvature via its lysophosphatidic acid acyltransferase activity. Here we report that a closely related isoform of Endo1, Endo3, inhibits clathrin-mediated endocytosis. Mutational analyses showed that the variable region of Endo3 is important in regulating transferrin endocytosis. In the brain, Endo3 is co-localized with dopamine D2 receptor in olfactory nerve terminals and inhibits its clathrin-mediated endocytosis in COS-7 cells. Furthermore, overexpression of Endo3 in an olfactory epithelium-derived cell line suppressed dopamine D2 receptor-mediated endocytosis and therefore accelerated its dopamine-induced differentiation. These results indicate that Endo3 may act as a negative regulator of clathrin-mediated endocytosis in brain neurons.

Annotation

Gene Ontology Annotations    

Cellular Component
presynapse  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Sh3gl2  (SH3 domain containing GRB2 like 2, endophilin A1)
Sh3gl3  (SH3 domain containing GRB2 like 3, endophilin A3)


Additional Information