RGD Reference Report - Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis.

Authors: Anggono, Victor  Smillie, Karen J  Graham, Mark E  Valova, Valentina A  Cousin, Michael A  Robinson, Phillip J 
Citation: Anggono V, etal., Nat Neurosci. 2006 Jun;9(6):752-60. doi: 10.1038/nn1695. Epub 2006 Apr 30.
RGD ID: 13504740
Pubmed: (View Article at PubMed) PMID:16648848
DOI: Full-text: DOI:10.1038/nn1695

Dynamin I is dephosphorylated at Ser-774 and Ser-778 during synaptic vesicle endocytosis (SVE) in nerve terminals. Phosphorylation was proposed to regulate the assembly of an endocytic protein complex with amphiphysin or endophilin. Instead, we found it recruits syndapin I for SVE and does not control amphiphysin or endophilin binding in rat synaptosomes. After depolarization, syndapin showed a calcineurin-mediated interaction with dynamin. A peptide mimicking the phosphorylation sites disrupted the dynamin-syndapin complex, not the dynamin-endophilin complex, arrested SVE and produced glutamate release fatigue after repetitive stimulation. Pseudophosphorylation of Ser-774 or Ser-778 inhibited syndapin binding without affecting amphiphysin recruitment. Site mutagenesis to alanine arrested SVE in cultured neurons. The effects of the sites were additive for syndapin I binding and SVE. Thus syndapin I is a central component of the endocytic protein complex for SVE via stimulus-dependent recruitment to dynamin I and has a key role in synaptic transmission.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Dnm1  (dynamin 1)
Pacsin1  (protein kinase C and casein kinase substrate in neurons 1)


Additional Information