RGD Reference Report - Changes in physicochemical parameters and alpha-crystallin expression in the lens during cataract development in OXYS rats. 5. - Rat Genome Database

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Changes in physicochemical parameters and alpha-crystallin expression in the lens during cataract development in OXYS rats. 5.

Authors: Rumyantseva, Yu V  Fursova, A Zh  Fedoseeva, L A  Kolosova, N G 
Citation: Rumyantseva YV, etal., Biochemistry (Mosc). 2008 Nov;73(11):1176-82.
RGD ID: 13503352
Pubmed: PMID:19120020   (View Abstract at PubMed)

The pathogenesis of cataract is associated with oxidative stress and with altered crystallin expression but it is still understood incompletely. In this study, the senescence-accelerated OXYS rats were used as a model. The first biomicroscopic signs of cataract in OXYS rats were registered at the age of 1.5 months; at 3 months morbidity reached 90%, and at 6 months it reached 100%. Cataract manifestation progresses: at 24 months mature cataract was detected in 90% of eyes of OXYS rats, whereas in 80% of Wistar rat eyes only initial signs of this disease were detected. Analysis of lens redox-parameters has shown that in OXYS rats the intensity of tryptophan fluorescence is higher, the GSH content being higher at 2 months but during formation of mature cataract at 13, 18, and 24 months being lower than in Wistar rats. Decrease in solubility of OXYS rat lens proteins was observed at the age of 13 months. At the age of 3 months gene expression of alphaA-crystallin and alphaB-crystallin was 3-fold and 25% lower, respectively, than in Wistar rats. At the age of 14 months there was a 27-fold decrease in expression of alphaB-crystallin in OXYS rats and it became 21-fold lower than in control. Proteins are synthesized in lens epithelial cells and dystrophic changes in senile cataract result in decrease in structural protein expression. The changes observed in OXYS rats are evidently associated with the dystrophic changes in lens epithelium, which we have described earlier, and are consistent with the model of senile cataract.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cataract  ISOCryaa (Rattus norvegicus)13503352; 13503352protein:decreased expression:lens:RGD 
cataract  ISOCryab (Rattus norvegicus)13503352; 13503352protein:decreased expression:lens:RGD 
cataract  IEP 13503352; 13503352protein:decreased expression:lens:RGD 
cataract MODEL: age-relatedIAGP 13503352 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cryaa  (crystallin, alpha A)
Cryab  (crystallin, alpha B)

Genes (Mus musculus)
Cryaa  (crystallin, alpha A)
Cryab  (crystallin, alpha B)

Genes (Homo sapiens)
CRYAA  (crystallin alpha A)
CRYAB  (crystallin alpha B)

Strains
OXYS/Nov  (NA)


Additional Information