RGD Reference Report - Adenovirus-mediated E2F-1 gene transfer in nonsmall-cell lung cancer induces cell growth arrest and apoptosis. - Rat Genome Database

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Adenovirus-mediated E2F-1 gene transfer in nonsmall-cell lung cancer induces cell growth arrest and apoptosis.

Authors: Kuhn, H  Liebers, U  Gessner, C  Schumacher, A  Witt, C  Schauer, J  Kovesdi, I  Wolff, G 
Citation: Kuhn H, etal., Eur Respir J. 2002 Sep;20(3):703-9.
RGD ID: 13464343
Pubmed: PMID:12358350   (View Abstract at PubMed)

Since overexpression of E2F-1 has been shown to induce apoptosis, the ability of adenovirus-mediated transfer of E2F-1 to inhibit tumour growth in nonsmall-cell lung cancer cell lines was investigated. Three cell lines with various genomic status were infected with AdE2F. Cell proliferation and viability were determined by trypan blue exclusion. Apoptosis induction was assessed by flow cytometry and poly-adenosine diphosphate-ribose-polymerase cleavage assay. In vivo, the effect of E2F-1 on tumour growth was determined in severe combined immunodeficiency (SCID) mice. The current experiments showed that overexpression of E2F-1 suppressed tumour cell growth. The population of apoptotic cells was dramatically increased 96 h after infection with AdE2F. Inhibition of cell growth and induction of apoptosis was not dependent on genomic status. Moreover, treatment of implanted tumours in SCID mice with AdE2F inhibited tumour growth. These data suggest that adenovirus-mediated E2F-1 gene therapy may be effective in the treatment of nonsmall-cell lung cancer.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lung non-small cell carcinoma treatmentIMP 13464343 RGD 
lung non-small cell carcinoma treatmentISOE2F1 (Homo sapiens)13464343; 13464343 RGD 

Objects Annotated

Genes (Rattus norvegicus)
E2f1  (E2F transcription factor 1)

Genes (Mus musculus)
E2f1  (E2F transcription factor 1)

Genes (Homo sapiens)
E2F1  (E2F transcription factor 1)


Additional Information