RGD Reference Report - A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats. - Rat Genome Database

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A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats.

Authors: Isotani, Ayako  Matsumura, Takafumi  Ogawa, Masaki  Tanaka, Takahiro  Yamagata, Kazuo  Ikawa, Masahito  Okabe, Masaru 
Citation: Isotani A, etal., Biol Reprod. 2017 Jul 1;97(1):61-68. doi: 10.1093/biolre/iox066.
RGD ID: 13464336
Pubmed: PMID:28859281   (View Abstract at PubMed)
DOI: DOI:10.1093/biolre/iox066   (Journal Full-text)

Acrosin, the trypsin-like serine protease in the sperm acrosome, was long viewed as a key enzyme required for zona pellucida penetration to fertilize eggs. However, gene disruption experiments in mice surprisingly showed that acrosin-disrupted males were fertile. Thus, the acrosin was considered to be not an essential enzyme for fertilization in mice. However, the involvement of acrosin in fertilization has been suggested in various species such as rat, bull, and pig. Moreover, it has been reported that serine protease (including acrosin) activity in mice is significantly weaker compared to other species, including rats. We analyzed the role of acrosin by disrupting the rat acrosin gene. It was found that, unlike in mice, acrosin was almost the sole source of serine protease in rat spermatozoa. Nevertheless, the acrosin-disrupted males were not infertile. However, the litter size from acrosin-disrupted males was decreased compared to heterozygous mutant rats. Further investigation using an in vitro fertilization system revealed that the acrosin-disrupted spermatozoa possessed an equal ability to penetrate the zona pellucida with wild-type spermatozoa, but the cumulus cell dispersal was slower compared to wild-type and heterozygous spermatozoa. This delay was presumed to be the cause of the small litter size of acrosin-disrupted male rats.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AcrRatacrosome matrix dispersal  IMP  RGD 
AcrRatpenetration of zona pellucida  IMP  RGD 
AcrRatprotein catabolic process  IMP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AcrRatdecreased litter size  IMP  RGD 
W;Cg-Acrtm1OsbRatdecreased litter size  IMP  RGD 
AcrRatimpaired acrosome reaction  IMP  RGD 
W;Cg-Acrtm1OsbRatimpaired acrosome reaction  IMP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Acr  (acrosin)

W;Cg-Acrtm1Osb  (Acr KO, Acr {tm1 Osb})

Additional Information