RGD Reference Report - Recognition of the tryptophan-based endocytosis signal in the neonatal Fc Receptor by the mu subunit of adaptor protein-2. - Rat Genome Database

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Recognition of the tryptophan-based endocytosis signal in the neonatal Fc Receptor by the mu subunit of adaptor protein-2.

Authors: Wernick, Naomi L B  Haucke, Volker  Simister, Neil E 
Citation: Wernick NL, etal., J Biol Chem. 2005 Feb 25;280(8):7309-16. doi: 10.1074/jbc.M410752200. Epub 2004 Dec 14.
RGD ID: 13461857
Pubmed: PMID:15598658   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M410752200   (Journal Full-text)

Endocytosis of membrane proteins is typically mediated by signals present in their cytoplasmic domains. These signals usually contain an essential tyrosine or pair of leucine residues. Both tyrosine- and dileucine-based endocytosis signals are recognized by the adaptor complex AP-2. The best understood of these interactions occurs between the tyrosine-based motif, YXXPhi, and the mu2 subunit of AP-2. We recently reported a tryptophan-based endocytosis signal, WLSL, contained within the cytoplasmic domain of the neonatal Fc receptor. This signal resembles YXXPhi. We have investigated the mechanism by which the tryptophan-based signal is recognized. Both interaction assays in vitro and endocytosis assays in vivo show that mu2 binds the tryptophan-based signal. Furthermore, the WLSL sequence binds the same site as YXXPhi. Unlike the WXXF motif, contained in stonin 2 and other endocytic proteins, WLSL does not bind the alpha subunit of AP-2. These observations reveal a functional similarity between the tryptophan-based endocytosis signal and the YXXPhi motif, and an unexpected versatility of mu2 function.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ap2m1Ratprotein binding  IPIFcgrt (Rattus norvegicus) RGD 
FcgrtRatprotein binding  IPIAp2m1 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ap2m1  (adaptor related protein complex 2 subunit mu 1)
Fcgrt  (Fc gamma receptor and transporter)


Additional Information